ABSTRACT

Basic Information

Abstract Number: 920 - 4
Author Name: Colin S Creaser - Loughborough University
Session Title: ACS ANYL - Ion Mobility Spectrometry in Pharmaceutical Analysis
Event Type: Symposia
Event Title: Applications of Microscale FAIMS Combined with Mass Spectrometry in Pharmaceutical Analysis

Presider Name:Alexander A Shvartsburg
Affiliation:PNNL

Date: Tuesday, March 19, 2013
Start Time: 09:15 AM (Slot #4)
Location: 201A

Abstract Content

In microscale field asymmetric waveform ion mobility spectrometry (FAIMS), ions are separated by their differential mobility as they travel through channels with sub-millimetre dimensions under the influence of an asymmetric dispersion field (DF). The narrow electrode gap allows substantially higher DF fields and faster separation than macroscale differential mobility devices. Separation on sub-second timescales becomes possible, allowing the device to be used during real-time LC-MS analysis. The performance of a microscale FAIMS-TOF system has been evaluated for pharmaceutical applications including metabolite and peptide determination in biofluids, metabolite profiling and the monitoring of potentially genotoxic impurities.

The LC-FAIMS-MS analysis is carried out by either by setting the FAIMS device to a fixed DF and compensation field (CF) to transmit a target analyte for quantitative analysis, or the CF may be scanned rapidly within the timescale of an HPLC peak. With FAIMS pre-selection, good linearity is observed over a wide dynamic range, with an improvement in signal-to-noise ratio and LOQ because FAIMS discriminates against chemical noise. FAIMS pre-selection may also be combined with in-source CID in the mass spectrometer interface in a tandem FAIMS-CID-MS approach, termed FISCID-MS. A FAIMS-selected precursor ion is fragmented and the fragments analysed by the mass spectrometer. LC-FISCID-MS aids the qualitative identification of analytes and can enhance selectivity in quantitative analyses.

The results of the performance evaluation demonstrate that microscale FAIMS combined with mass spectrometry is a qualitative and quantitative technique applicable to both small and large molecules that can provide improved levels of analytical performance compared to mass spectrometry alone.