ABSTRACT

Basic Information

Abstract Number: 260 - 6
Author Name: Donald F Hunt - University of Virginia
Session Title: Pittsburgh Conference Achievement Award
Event Type: Awards
Event Title: Innovative Technology for the Identification of Post-Translational Modifications - New Findings in Research Related to Plant Biology, Eoigenetics and Immunotherapy of Cancer

Presider Name:Jane Chan
Affiliation:Bechtel Bettis, Inc.

Date: Monday, March 18, 2013
Start Time: 09:55 AM (Slot #6)
Location: 114

Abstract Content

This lecture will focus on a combination of enrichment technology, chemical derivatization and mass spectrometry instrument development that facilitates identification of posttranslational modifications on proteins. Development of a new, front-end ETD ion source that facilitates simultaneous generation of positive and negative ions by electrospray ionization and chemical ionization, respectively, now makes it possible to record collision activated dissociation (CAD) and electron transfer dissociation (ETD) mass spectra on high resolution LTQ-FT and orbitrap instruments. Parent ion and fragment ion mass spectra can be obtained at resolutions in excess of 40,000 and with mass measurement accuracy in the low ppm range. Application of this technology to the identification of post-translational modifications on plant transcription factors, on mammalian centromeric chromatin, and on MHC peptides that are uniquely presented by cancer cells to the immune system will be described.