ABSTRACT

Basic Information

Abstract Number: 260 - 7
Author Name: Scott McLuckey - Purdue University
Session Title: Pittsburgh Conference Achievement Award
Event Type: Awards
Event Title: Bioconjugation in the Gas Phase: New Chemistry for Tandem Mass Spectrometry

Presider Name:Jane Chan
Affiliation:Bechtel Bettis, Inc.

Date: Monday, March 18, 2013
Start Time: 10:30 AM (Slot #7)
Location: 114

Abstract Content

The advent of ionization methods that form multiply charged ions from biomolecules has allowed for the exploration of ion/ion reactions as a new class of chemical reactions in analytical mass spectrometry. Attractive features common to all ion/ion reactions are that they are highly exothermic for virtually any anion/cation combination and that the overall reaction kinetics are independent of the chemical identities of the reactants. The ion/ion reactions of multiply charged peptide, protein, and oligonucleotide ions involving proton transfer, electron transfer, or metal ion transfer have been extensively studied. Recent results have shown that much more complex types of reaction that involve bond formation and bond cleavage can also take place during the course of an ion/ion reaction. These results demonstrate that selective chemistries can be effected between stages of mass spectrometry. Some of these reactions are those normally associated with bio-conjugation chemistries in solution.

This presentation will summarize current understanding of the dynamics of gas-phase bio-ion/ion reactions with emphasis on the factors that determine the major observed pathways. Phenomena common to all such reactions will be described first followed by the implications for particular reaction types. Of particular emphasis in this presentation will be electron transfer, single proton transfer, multiple proton transfer is a single ion/ion encounter, metal transfer, and attachment. Essentially all of these outcomes can be dominant for a given analyte, depending upon the form of the analyte ion and the identity of the reagent. This presentation will draw on data, both old and new, that illustrate the underlying factors that make reagents ions either particularly well-suited or poorly suited for a particular analyte ion transformation of interest.