Fast Liquid Chromatography is rapidly gaining acceptance in laboratories performing routine analysis. In order to increase throughput, conventional LC methods have been transferred to Fast LC methods through the use of small particle size (sub 2um) columns operated at elevated pressures.
Transferring a method from conventional to fast-LC environment requires special considerations and a rigorous analytical approach. Column particle size and geometry, instrumental bandwidth, mobile phase flow rate, composition, gradient profile and temperature are only some of the variables that need to be taken in consideration. Once the analytical conditions and instrument set up are defined, a method validation protocol must be applied to the new fast-LC in order to verify accuracy and precision, before it can be applied to routine use.
This study will critically evaluate the porting an analytical method from conventional to fast-LC. Conventional and fast-LC methods developed for the determination of flavor, preservative and additive ingredients in beverage products and for the determination of toxic impurities in consumer products will provide practical examples to illustrate the challenges and best practices involved. Results of method validation protocols will be statistically evaluated using ANOVA, the Dixon test and other chemometric tools to confirm validity of results, and will provide an objective measure of the extent to which the fast-LC method transfer has met the target data quality objectives.
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