Course Information
Course Title: LC/MS: Method Development and Applications to Small Molecules
Categories: 1 - Atmospheric Pressure Ionization
2 - Life Sciences
3 - Liquid Chromatography
4 - Liquid Chromatography-Mass Spectrometry
5 - Mass Spectrometry
6 - Pharmaceutical Sciences
Instructor(s): Guodong Chen Course Number: 75
Affiliation: Bristol-Myers Squibb
Course Date: 03/14/2012 - Wednesday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $455 ($655 after 2/13/12) Textbook Fee:

Course Description
This course is designed to be an introduction to the theory and practical implementation of LC/MS and LC/MS/MS technology in the laboratory. It emphasizes problem-solving skills with examples encountered in industrial and academic research including characterization of trace level drug substance impurities and degradation products, identification of drug metabolites, and the analysis of natural products. The interpretation of mass spectra will be illustrated with practical examples. This course will focus on atmospheric pressure ionization interfaces including electrospray and atmospheric pressure chemical ionization, and will survey the various mass analyzer options. A thorough coverage of approaches toward method development for qualitative analysis of pharmaceutical products will provide a good starting point for understanding the practical issues facing implementation of LC/MS in the laboratory.

Target Audience
This course is designed for practicing analytical scientists (new users, chromatographers, analytical chemists, and laboratory managers) performing/supporting small molecule analysis, in-process testing, quality control, quality assurance, research and development, and manufacturing.

Course Outline
TOPICS
►Introductions to Liquid Chromatography (LC)/Mass Spectrometry (MS)
• History
• Ionization
• Atmospheric Pressure Ionization API
►Mass Analyzers
• Quadrupoles
• Ion Trap
• Ion Cycotron Resonance (ICR)
• Time-Of-Flight
• Orbitrap
• Tandem Mass Spectrometry (MS/MS) and LC/MS/MS
►LC/MS Method Development
• Issues / Sample Preparation
• Electrospray Ionization (ESI)
• Atmospheric Pressure Chemical Ionization (APCI)
• Automation and High Throughput Sample Analysis
►Applications—Small Molecules Structure Elucidation
• Impurities
• Degradation Products
• Metabolites
• Natural Products
• Unknowns

Course Instructor's Biography
Dr. Guodong Chen has extensive pharmaceutical research experience in major pharmaceutical companies, including Eli Lilly, Schering-Plough (now Merck) and Bristol-Myers Squibb. He is currently heading a mass spectrometry group at Bristol-Myers Squibb’s Princeton site, providing mass spectrometric/analytical support to drug discovery and development programs in small molecule pharmaceuticals and biologics. He has over 55 research publications in peer-reviewed journals and book chapters covering the broad area of mass spectrometry and analytical sciences. He co-edited two books on “Characterization of Impurities and Degradants Using Mass Spectrometry” and “Protein and Peptide Mass Spectrometry in Drug Discovery” (both by Wiley). He teaches short courses at EAS and Pittcon. He is a frequently invited speaker at conferences and academic institutes with over 70 presentations. He also organized/chaired scientific sessions at various forums, including major sessions on mass spectrometry of small molecule pharmaceuticals and biologics at EAS, Pittcon, ASMS conference and ACS meetings. Dr. Chen was the Chairperson of the North Jersey Section of ACS Mass Spectrometry Discussion Group (2004) and in 2006, he received Early Career Award in Mass Spectrometry. He was an invited Analytical Chemistry Program Chair for ACS MARM Conference (2005) and served as President of Chinese American Chemical Society-Tri State (2007). Dr. Chen received his Ph.D. in Analytical Chemistry from Purdue University under the direction of Professor R. Graham Cooks.