Course Information
Course Title: Preparative HPLC - Fundamentals and Applications
Categories: 1 - Liquid Chromatography
2 - Pharmaceutical Sciences
3 - Sample Preparation
4 - Food
Instructor(s): Fred Rabel Course Number: 34
Affiliation: ChromHELP, LLC
Course Date: 03/14/2012 - Wednesday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $235 ($335 after 2/13/12) Textbook Fee:

Course Description
This course covers the basics of beginning any scale-up of a HPLC separation based on an existing analytical method. The needs may be a few milligrams to grams of a component depending on the stage of development. The approach also depends on the complexity of the sample - few or many components? Effects on increasing the sample size on an analytical system are discussed since this is often the first step in scaleup. The needs are then evaluated to see how to proceed with either larger columns or systems, and the cost to accomplish this. The emphasis will be on preparative HPLC using the reversed phase mode. Selected applications from the literature of scaling up a separation will be discussed.

Target Audience
The target audience for this course is anyone who has done HPLC but now needs the knowledge to begin the scaling up for collection of components for further study. This course will allow them to collect their desired components with a minimum of problems and cost.

Course Outline
• Preparative HPLC - what it is
• Comparison of analytical and preparative separations
• Effects of overloading the LC column
• Solving mass or volume overloading problems
• Scaling up the separation

Course Instructor's Biography
Fred Rabel is the President of ChromHELP, LLC, consulting and teaching all of the liquid chromatography techniques (TLC, HPLC, Column Chromatography, Sample Preparation). He previously worked for J.T.Baker Chemical Co., Whatman Inc., and EMD Chemicals. He is on the editorial boards of Journal of Liquid Chromatograpnhy and Related Techniques, and Journal of Chromatographic Science. He has been teaching HPLC courses for over 35 years. He has authored many articles and chapters in chromatography.