Course Information
Course Title: Essentials of Modern HPLC 2 and UHPLC: Practice, Operation, Troubleshooting and Method Development
Categories: 1 - Life Sciences
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Pharmaceutical Sciences
5 - Quality/Regulatory/Compliance
6 - Environmental Analysis
Instructor(s): Michael Dong Course Number: 6
Affiliation: Genentech
Course Date: 03/11/2012 - Sunday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $455 ($655 after 2/13/12) Textbook Fee: $65 (same text as course #3)

Course Description
This course will introduce you to best practices of high-performance liquid chromatography (HPLC) as well as tricks-of-the-trade for successful HPLC operation. The attendees will learn step-by-step guide to operating HPLC modules, maintenance procedures, troubleshooting strategies, traditional and accelerated method development processes as well as recent advances of this pervasive analytical technique including ultra-high-pressure LC. This is the second part of a two-course series to Modern HPLC introduction. “Essentials of Modern HPLC 1” introduces HPLC fundamental concepts, columns, instrumentations and applications, is a prerequisite for “Essentials of Modern HPLC 2” for beginners.

Target Audience
Analysts, scientists, researchers and managers who want to get an updated introduction of modern HPLC practices, troubleshooting, method development and recent advances (UHPLC). You should have a good understanding of general chemistry. Some prior hands-on HPLC experience would be helpful.

Course Outline
Program Agenda 
1. HPLC operation

• Safety and environmental concerns

• Mobile phase preparation

• Best practice in HPLC operation

• Guidelines for increasing HPLC precision and for trace analysis

• Qualitative and quantitative strategies

2. HPLC maintenance and troubleshooting

• Common HPLC maintenance procedures

• Problem diagnosis and troubleshooting guide

• Diagnosing and solving problems (pressure, baseline, peak, data performance)

• Case studies

3. HPLC method development and validation
  
• Tradition strategy for method development

• 3-pronged template approach for efficient method development

• Method prequalification and validation

• Modern trends – software tools and automated systems

4. Novel approaches and Ultra-high-pressure LC

• High-purity silica, novel bonding chemistries, and hybrids.

• HILIC, monolith, sub-3mm and sub-2 mm packing, fused core particles

• Ultra high-pressure LC – performance, perspectives and potential issues

Course Textbook Recommended!
M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Course Instructor's Biography
Dr. Michael W. Dong is a Senior Scientist at Genentech, Small Molecule Drug Discovery, South San Francisco, CA. He was formerly Research Director at Synomics Pharma, Research Fellow at Purdue Pharma, Senior Staff Scientist at Applied Biosystems/ Perkin-Elmer, and section-head in Hoechst Celanese. He holds a Ph.D. in Analytical Chemistry from City University of New York, and recently completed a certificate in Biotechnology at U. C. Santa Cruz. He has conducted training courses at national meetings (ACS, Pittcon, EAS, AAPS) on HPLC in pharmaceutical analysis and DMPK, HPLC method development, ultra-high-pressure LC and drug development process for chemists. He has over 80 publications in chromatography and analytical chemistry. He authored a best-seller in chromatography - Modern HPLC for Practicing Scientists, Wiley, 2006 and co-edited Handbook of Pharmaceutical Analysis by HPLC, Elsevier/Academic Press, 2005.