Course Information
Course Title: Ion Mobility Spectrometry
Categories: 1 - Mass Spectrometry
2 - Pharmaceutical Sciences
3 - Clinical Analysis
4 - Proteomics
5 - Homeland Defense
6 - Ion-Mobility Spectrometry
Instructor(s): Herbert Hill, Bill Siems Course Number: 94
Affiliation: Washington State University
Course Date: 03/13/2012 - Tuesday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $235 ($335 after 2/13/12) Textbook Fee:

Course Description
This course is a comprehension overview of theory and application of all types of ion mobility spectrometry. Ion Mobility Spectrometry (IMS) has come of age in the 21st century as an analytical detection and separation method. Applications of IMS and IMS - Mass Spectrometry (IMS-MS) are expanding rapidly – from sensors for explosive and chemical warfare agent detection to rapid methods for separation of complex biological mixtures before MS. Today, there are a number of commercial IMS instruments available of various designs and objectives. Airports use IMS instruments for detecting explosives and government agencies carry IMS instruments on raids for on-site identification of drugs of abuse. On-site monitoring of pesticides, chemical warfare agents and industrial chemicals are common. IMS is no longer limited to gas phase samples as the introduction of electrospray as an ionization source has enabled IMS to be used for the analysis of aqueous samples. Development of high-resolution ion mobility spectrometry has led to the rapid separation of complex mixtures and isomeric compounds. Extension of IMS as a tool for rapid analysis into areas such as drug discovery and molecular biology is truly exciting. This basic course introduces the analytical applications of ion mobility for separation and measurement of a wide variety of chemicals. The primary objective of the course will be to offer sufficient theoretical and practical information to evaluate IMS for real-world applications. Discussions of instrumental designs, ionization sources, modes of operation, chemical interferences, resolution, sensitivity, detection limits, and dynamic response ranges will provide a knowledge base from which to select an instrument and method that will best accomplish a desired measurement. In addition, we will discuss methods for measuring the size of ions by IMS and when coupled to mass spectrometry “ion density” can be measured and used to identify classes of compounds.

Target Audience
The target audiences are scientists from pharmaceutical and industrial companies as well as government agencies who have recently purchased or who are considering purchasing an ion mobility spectrometer or an ion mobility spectrometer coupled to a mass spectrometer.

Course Outline
Session I:  Ion Mobility Spectrometry
1. Introduction
o Overview of applications
2. Types of Ion mobility spectrometry
o Time-of-flight
 Ambient pressure
 Low pressure
o Field Asymmetric Ion Mobility Spectrometry
o Deferential Mobility Spectrometry
o Aspirating Ion Mobility Spectrometry
o Hyphenated Methods
 IMS-DMS
 IMS-FAIMS
 DMS-IMS
 FAIMS-IMS
3. Ionization Sources used in IMS
o Radioactive sources
o Corona discharge 
o Photo ionization 
o Electrospray ionization
4. Figures of Merit
o Sensitivity
o Detection limit
o Dynamic response range
o Selectivity
o Resolving power
5. Parametric affects on Figures of Merit
o Temperature
o Pressure
o Electrical potential
o Geometry
6. Applications
o Explosives detection
o Chemical warfare agent detection
o Drug detection
o Drug clean-up validation
o Environmental analysis
Session II: Ion Mobility coupled with Mass Spectrometry
1. Review of Ion Mobility Spectrometry
a. Time-of-flight Ion Mobility Spectrometry
b. Field Asymmetric Ion Mobility Spectrometry
2. Types of Ion Mobility Mass Spectrometry
a. IMS-Quad MS
b. IMS-Ion Trap MS
c. IMS-TOFMS
i. Electrospray ionization
1. low pressure ion mobility 
2. Ambient pressure ion mobility
ii. MALDI
3. Applications
a. Proteomics
b. Metabolomics
c. Glycomics
d. Gas Phase Chiral Separations
e. Security
i. Explosives
ii. Chemical warfare agents
iii. Biological warfare agents
f. Environmental analysis

Course Instructor's Biography
Dr. Herbert H. Hill, Jr. is a Regents Professor at Washington State University where he teaches in the Chemistry Department. He serves as one of the editors of the International Journal of Ion Mobility Spectrometry and on the Editorial Advisory Board of the International Journal of Mass Spectrometry. He obtained his Ph.D. degree in Chemistry in 1975 from Dalhousie University of Halifax, Nova Scotia, under the direction of W.A. Aue. While at Dalhousie, he was a member of the Trace Analysis Research Centre and was awarded the Izaak Walton Killam Memorial Scholarship for advanced study. Before joining the chemistry faculty at WSU in 1976, he was a postdoctoral fellow in the laboratory of F. W. Karasek at the University of Waterloo where he helped first develop ion mobility spectrometry for use in analytical chemistry. In the 1980’s he received a Japan Society for the Promotion of Science (JSPS) fellowship for IMS and the Keene P. Dimick award for the development of electrospray ion mobility spectrometry. Throughout the 1990s and 2000s he continued to develop IMS using electrospray ionization for the detection of chemical warfare agents, explosives and drugs as well as developing high resolution IMS coupled with mass spectrometry for the separation and detection of biological compounds. In recent years his interest has been in the determination of interferences to the IMS detection of explosives, investigating the accurate measurement of collision cross sections by IMS and the separation, detection and identification of biochemicals in complex biological samples, including the gas phase separation of chiral compounds. He has over 250 peer reviewed scientific publications; numerous patents; and two edited books on trace detection and has authored two books on ion mobility and ion mobility mass spectrometry due to be published in 2012. At Washington State University he has served as director of the Office of Grants and Research Development and Associate Dean of the Graduate School. He was recently selected as the Distinguished Faculty of Science at Washington State University.