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Short Course

Course Information
Course Title: Biosensors for Food Safety: Immunoassay Design and Detection Techniques
Categories: 1 - Quality/Regulatory/Compliance
2 - Sensors
3 - Food
4 - Spectroscopy
5 - Water-Wastewater
Instructor(s): Betsy Yakes Course Number: 180
Affiliation: U.S. Food and Drug Administration
Course Date: 03/18/2013 - Monday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $260 ($360 after 2/18/13) Textbook Fee:

Course Description
Rapid and sensitive detection of pathogens, contaminants, and constituents is essential to food safety and food quality control. Immunoassays are a highly successful technique for the detection of analytes, and recent advances in detection have improved upon the sensitivity, speed, and reliability of monitoring foodstuffs. This course will provide overviews of chip-based immunoassays and common detection methods. Additionally, a surface plasmon resonance (SPR) biosensor for food pathogen detection will be used as a model system to teach principles of assay design as well as common challenges associated with detecting analytes from food matrices.

Target Audience
This short course is designed for anyone who currently performs, or will be learning to perform, immunoassays as well as those that use surface plasmon resonance (SPR) instrumentation. Those interested in general biosensor applications for food safety will also benefit from the course content.

Course Outline
1. Overview of immunoassays
2. Introduction to readout methods
3. Food applications of biosensors
4. Comparison of readout techniques for food pathogen detection
5. Overview of surface plasmon resonance biosensors
6. Assay design: biosensor surfaces, binding interaction optimization, regeneration, and data analysis considerations

Course Instructor's Biography
Dr. Betsy Jean Yakes is currently an analytical chemist specializing in immunoassay development and spectroscopic techniques for food contaminant detection at the U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition. Her research interests include developing immunoassays using novel instrumentation for improved quantitation of bacterial and viral pathogens as well as small molecule and protein toxins. Her early research at Iowa State University focused on the development of surface-enhanced Raman spectroscopy biosensors which led to a patented bioamplification technique for microbial signal transduction.