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Short Course

Course Information
Course Title: Essentials of Modern HPLC/UHPLC 1: Fundamentals and Applications
Categories: 1 - Liquid Chromatography
2 - Liquid Chromatography-Mass Spectrometry
3 - Pharmaceutical Sciences
4 - Quality/Regulatory/Compliance
5 - Environmental Analysis
6 - Food
Instructor(s): Michael Dong Course Number: 1
Affiliation: Genentech
Course Date: 03/16/2013 - Saturday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $480 ($680 after 2/18/13) Textbook Fee: $65 (Same text for Course #2)

Course Description
This course will provide you with an updated overview and a solid working knowledge of high-performance liquid chromatography (HPLC) and Ultra-high-pressure LC (UHPLC). The attendees will learn useful theoretical concepts, instrumentalfundamentals and operating principles, columnbasics and selection guide, and key applications in various industries. This is the first part of a two-courseseries forEssential of Modern HPLC/UHPLCintroduction conducted at an intermediate level.The second part, “Essential of Modern HPLC/UHPLC 2”,is a follow-on course that focuses on thepractice of modern HPLC:operation, maintenance, troubleshooting, method developmentas well as performance, practice and potential issues of UHPLC.

Target Audience
Analysts, scientists, researchers, and managers who want to get an updated introduction of modern HPLC fundamentals and its diversified applications. Topics covered included essential concepts, HPLC columns, instrumentation, and practical applications. The focus is to provide the attendees with a concise overview of this common analytical technique and how to apply it to solve practical problems. It is recommended that you have a good understanding of general chemistry. Some prior hands-on HPLC experience would be helpful. This course is conducted at the intermediate level.

Course Outline
1. Introduction and fundamental concepts
Corollaries and common “faux pas”
History, advantages, limitations, and modes
Retention time (tR), retention factor (k), separation factor (a), column efficiency (N), column void volume (VM), and resolution (Rs).
Mobile phase factors (organic modifiers, pH, buffers), operating parameters (Flow, Gradient time (tG), column temperature (T)), and peak capacity (Pc)

2. HPLC columns, trends and selection guides
Column characteristics and types, packing characteristics (support type, particle size, pore size)and bonding chemistries.
Trends of shorter and narrower columns packed with small particles, high-purity silica, novel bonding chemistries.
Van Deemter equation.
Column selections guide.
HILIC, monoliths, hybrids, sub-3, sub-2 mm and fused core columns.

3. HPLC instrumentation and operating principles
Solvent delivery system, injector, autosampler, detector (UV/Vis, photodiode array, fluorescence, refractive index, ELSD, CAD, conductivity, and mass spectrometer (MS), and data handling system.
HPLC system calibration
Concepts and ramifications of dwell volume and instrumental bandwidth (extra-column bandbroadening).


4. Practical applications of HPLC/UHPLC in diversified industries
An overview of HPLC applications in diversified industries supported with specific case studies.
Pharmaceutical: drug discovery to quality control, assay, impurities, chiral separations, PKDMand dissolution.
Food: sugars, fats, organic acids, and additives.
Environmental: US EPA methods, pesticides, and PAHs.
Chemical:GPC,plastics, and ion-chromatography.
Bioseparations and life sciences: proteins, peptides, peptide mapping, amino acids, oligonucleotides, nuclei acids, and PCR products.

Course Instructor's Biography
Dr. Michael W. Dong is a Senior Scientist at Genentech, Small Molecule Drug Discovery, South San Francisco, CA. He is responsible for new technologies, automation and supporting multiple late-stage research projects in Small Molecule Analytical Chemistry and QC, Small Molecule Pharmaceutical Sciences. He was formerly Research Director at Synomics Pharma, Research Fellow at Purdue Pharma, Senior Staff Scientist at Applied Biosystems / Perkin-Elmer, and section-head in Hoechst Celanese. He holds a Ph.D. in Analytical Chemistry from City University of New York, and a certificate in Biotechnology at U. C. Santa Cruz. He has conducted training courses at national meetings (ACS, Pittcon, EAS, and HPLC) on HPLC in pharmaceutical analysis and DMPK, HPLC method development, ultra-high-pressure LC, drug development process for scientists, and drug quality fundamentals. He has over 80 publications in chromatography and analytical chemistry. He authored a best-seller in chromatography - Modern HPLC for Practicing Scientists, Wiley, 2006 and co-edited Handbook of Pharmaceutical Analysis by HPLC, Elsevier/Academic Press, 2005. He is an editorial advisory board member of LC.GC magazine and American Pharmaceutical Review.