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Short Course

Course Information
Course Title: Solid-State Light Sources: Utilize the Benefits of Light Emitting Diodes and Laser Diodes as the Light Sources of the 21st Century
Categories: 1 - Life Sciences
2 - Capillary Electrophoresis
3 - Flow Injection Analysis
4 - Sensors
5 - Teaching Analytical Chemistry
6 - Laboratory-on-a-chip/Microfluidics
Instructor(s): Mirek Macka Course Number: 145
Affiliation: University of Tasmania
Course Date: 03/16/2013 - Saturday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $480 ($680 after 2/18/13) Textbook Fee:

Course Description
The course will give an intensive introduction to the principles, technology, properties and practical usage of Solid-State Light Sources (SSLSs) - Light Emitting Diodes (LEDs) and Laser Diodes (LDs). The participants will gain the necessary theoretical and practical knowledge to understand and utilize the immense potential of SSLSs. The emphasis will be on learning how to select, characterize and use LEDs and LDs for a wide variety of applications in optical detection and visualization, chemistry and life sciences in general, and teaching. Examples will be given with guidance for hands-on experiments. The course participants will receive free of charge a color printed workbook based on over 350 slides.

Target Audience
A wide range of participants will benefit, including scientists, engineers, technicians, and students, including those currently only considering whether they could use LEDs and LDs in their work, and further those educators involved in teaching of instrumental analytical chemistry, physical chemistry, photochemistry and other areas utilizing light sources.

Course Outline
• Opening & introductions, participants’ expectations, structure, contents, time schedule.
• Why use SSLSs - LEDs and LDs - in chemistry and science?
• A brief history – how it came to SSLSs being hailed as ‘the light sources of the 21st century’?
• Fundamentals (physical principles, construction) – what should one know as a user?
• Basics of SSLSs in respect to their applications in analysis, chemistry and life sciences.
• Application areas: examples will include a wide range of usage from optical detection, optical sensors, microfluidics, visualization, fluorescence microscopy, photochemistry, photolithography, plant science, teaching, and other areas using light sources – attention will be paid to participants’ own interests.
• How to choose the right LED or LD?
• Practical issues of using SSLSs users should know of: powering, measuring optical characteristics (emission spectra, optical power output), connecting LEDs into arrays etc.
• Guidance to hands-on experiments: measuring emission spectra, optical power, intensity, transmittance, absorbance, pulsing LEDs and use of lock-in amplifier techniques.
• Coupling of SSLSs to optical fibers.
• Pulsed techniques: use of lock-in amplifiers and LEDs in TRF (time resolved fluorescence).
• Optical methods in chemical analysis with an emphasis on photometry and photometric detection in analytical flow-through methods (FIA, HPLC, CE), fluorimetry and optical detection methods in microfluidic chips, optical sensors, visualization, fluorescence microscopy.
• Fluorescence microscopy and visualization using inexpensive designs (less than $250)
• Basics of construction of simple LED-based photometers and fluorimeters.
• Diode lasers for compact inexpensive LIF (Laser Induced Fluorescence) detection in on capillary and microfluidic chip formats.
• LEDs as light sources for photochemistry including photopolymerisation of polymers such as porous polymer monoliths.
• Other advanced and specialized methods such as heating with SSLSs, numerical modeling etc.
• Workshop using examples from participants’ own specific usage, questions, discussion.

Course Instructor's Biography
Mirek Macka is a New Stars Professor at the University of Tasmania, Australia, where he earned his Ph.D. in Chemistry. He has published over 150 journal papers and book chapters on fundamentals and applications to the solutions of real world problems primarily in the area of chemical analysis and separation methods - capillary electrophoresis, liquid chromatography, miniaturized and microfluidic chip based analysis, and detection methods. He has pioneered the use of light emitting diodes in his research since 1995 and published extensively on the use of LEDs in optical detection methods and other areas of chemistry and life sciences.