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Short Course

Course Information
Course Title: Advanced Topics in Capillary Gas Chromatography – Getting More from your GC
Categories: 1 - Gas Chromatography
2 - Gas Chromatography-Mass Spectrometry
Instructor(s): Matthew Klee Course Number: 131
Affiliation: XO Associates, LLC
Course Date: 03/16/2013 - Saturday Course Length: 2 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Course Date 2: 03/17/2013 - Sunday    
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $925 ($1325 after 2/18/13) Textbook Fee:

Course Description
For experienced GC users who are curious about the latest trends and advancements in capillary gas chromatography. Overviews will provide straightforward explanations of the principles and practices associated with each topic. Attendees will be able to make informed choices between available options and then implement the sections in an efficient and effective manner.

Target Audience
Hands-on users of GC and GC/MS who face the challenges of optimizing methods, transferring old methods to new equipment, lower costs per analysis, or taking advantage of some of the new technologies that have been recently introduced.

Course Outline
Day 1

1. Capillary GC tune-up
a. Common myths and misconceptions debunked
b. Current best practices from sample introduction to data analysis
2. Large volume injection
a. Fundamental processes
b. Common approaches (PTV, COC)
c. Practical considerations
3. Auxiliary sampling and focusing
a. Samplers for gases, liquids, and solids
b. Concentrating processes and instrumentation
4. Fast GC – how to painlessly and quickly migrate your current methods
a. Compromises/tradeoffs
b. Concepts of method translation
c. Putting it together

Day 2

1. Multidimensional separations (instrumentation, practice)
a. Detection (multiple and multiplex)
b. Separations
i. Deans switch, heartcutting
ii. Comprehensive multidimensional (GC×GC)
2. Capillary column backflushing – BF with benefits
a. Three possible configurations
b. Two modes
c. Considerations for adding backflush to a current method
3. Automated sample preparation
a. Common sample preparation steps that are easily automated
b. Typical instrumentation used for automated sample preparation
4. Optimal mass spec.
a. Fundamental processes involved
b. Typical problems that arise
c. Troubleshooting and maintenance
5. Method development and troubleshooting - interactive discussion
a. How to describe a method so others can understand and reproduce
b. Your method problems discussed in class
iii. Underlying processes involved
iv. Potential impact of current setpoint choices on observed problem
v. Testing your hypotheses in most efficient manner (fastest way to an answer)

Course Instructor's Biography
Matthew S. Klee, Ph.D. is a recognized authority in the area of GC analysis and instrumentation. For 23 years at HP/Agilent he was directly involved in the development and marketing of market-leading GC and GC/MSD instrumentation. Dr. Klee is a prolific inventor and author in various areas of separation science, chemometrics, and spectroscopy. He is the lead author of the monthly gas chromatography e-journal called GC Solutions (published by Separations Science) wherein he publishes articles on the practical use of GC. He also presents GC Master Classes internationally in collaboration with Separation Science. Dr. Klee’s most recent developments and interests center on method optimization, instrument development. Capillary Flow Technology, capillary column backflushing, multidimensional separations and in making the practice of gas chromatography more accessible and understandable to its many practitioners.