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Short Course

Course Information
Course Title: It's Alive! The Rise of Protein Dynamics Analysis by Mass Spectrometry
Categories: 1 - Life Sciences
2 - Liquid Chromatography-Mass Spectrometry
3 - Mass Spectrometry
4 - Pharmaceutical Sciences
5 - Proteomics
Instructor(s): Michael Greig, Ben Bolanos Course Number: 168
Affiliation: Pfizer Oncology
Course Date: 03/03/2014 - Monday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $270 ($375 after 2/10/14) Textbook Fee:

Course Description
This half day course will introduce attendees to mass spectrometry based methods for analyzing protein dynamics. Proteins can rapidly undergo dramatic changes in primary and tertiary structure which are key to their cellular function. Understanding this flux in protein state is critical in understanding ways to modify or modulate protein behavior.  We will introduce a collection of mass spectrometry-based assays that were developed to better understand protein dynamics and these temporal affects on biological pathways. A discussion of structural and cellular dynamics will include:  how to utilize HDX MS data for detailed structural dynamic analysis, how to measure protein turnover rates in cellular systems, advanced methods in protein characterization and quantification, and ways to measure interactions within biomolecular complexes. More importantly, we will discuss why we need to incorporate protein dynamics into our daily research. 

Target Audience
The course is ideal for analytical chemists, biologists, Ph D students and Post-Docs in any branch of biological or life sciences, lab managers and group leaders, or anyone else interested in how mass spectrometry can play a role in analyzing protein dynamics. Knowledge of mass spectrometry is helpful, but not necessary.

Course Outline
Introduction to Protein Dynamics
Protein Characterization and Quantification
Hydrogen/Deuterium Exchange Mass Spectrometry (MS)
Non-Covalent Biomolecular MS
Protein Turnover Analysis
Conclusions/Discussion

Course Instructor's Biography
Mike Greig is an Associate Research Fellow and leads Protein Dynamics, a core biological mass spectrometry research group at Pfizer (La Jolla Labs). Prior to that, he worked at Revlon Science Institute as a polymer and analytical chemist and was the Mass Spectrometry Lab Manager at Isis Pharmaceuticals. He has over 20 years experience in the field of mass spectrometry and analytical chemistry, analyzing everything from small molecules to polymers to proteins. He has taught over 25 mass spectrometry classes at various companies and scientific conferences around the world, was a Keynote speaker at the International Mass Spectrometry Conference, a member of the Lab Automation Scientific Committee, current member of National High Magnetic Field Laboratory FTICR MS Advisory Panel, and has over 60 presentations and publications. Ben Bolanos is a Senior Principal Scientist in the Protein Dyanmics group at Pfizer (La Jolla, CA). He has been with Pfizer for over 12 years, where he has operated an array of mass spectrometers for drug discovery and design applications. Ben has a PhD in analytical chemistry from the University of Texas in 1997 - research focused on probing protein tertiary structure by electrospray/FT-ICR mass spectrometry. He did Post-doctoral research position at The Rowland Institute at Harvard - involved in the design of a quadrupole trap for studying noncovalent complexes. Ben was Co-Chair of the Metabolomics workshop at the ASMS Conference for 2005-2006, contributed to numerous presentations and publications, and taught several mass spectrometry classes at scientific conferences around the world.