Successful Integration of Fast LC with MS Detection
Tuesday, March 15th, 2011
2:00 PM Room: 409B

Organizers:
Philip J Koerner, Phenomenex

Speakers:
2:00 PMIntroductory Remarks -
2:05 PMFast Profiling of Biological Tissues and Fluids in the Context of Metabolic Research, Michael Lassman
2:35 PMRealizing Ultra-high Performance with UHPLC-MS/MS Using Core-shell Sorbents and Considerations for Instrument Optimization, A Carl Sanchez
3:05 PMImplementing Sub 2 um and Fused-core Particle Chromatography for Fast LC in the Regulated Bioanalytical Environment, Jennifer Cunliffe
3:35 PMRecess
3:50 PMHigh Sensitivity and Throughput for Dried Blood Spot Analysis Using Microflow LC Interfaced to Electrospray Ionization, Hesham Ghobarah
4:20 PMHigh Productivity Columns with LC/MS Applications, Maureen Joseph
4:50 PMDiscussion/Wrap Up


Overview:
Since their introduction several years ago, ultra high performance liquid chromatography (UHPLC) columns based on fully porous sub 2µm and, more recently, core-shell media have been widely used to take advantage of the significant improvements in chromatographic performance (efficiency and resolution) these columns deliver. The more tangible benefits provided by UHPLC columns have been in reducing analysis times and increasing sample throughput. The narrow chromatographic peak widths provided by UHPLC columns represent a significant and non-trivial challenge for integration with mass spectrometry (MS) detection. Monitoring multiple MRM’s requires careful consideration of MRM windows and implementation of acquisition programs. The papers presented in this workshop will highlight how the speed of UHPLC has been successfully integrated with MS to provide high speed, high sensitivity solutions for a variety of analytical challenges.