Basic Information

Abstract Number: 1770-5     Author Name: Norman Dovichi Affiliation: University of Washington Session Title: Young Investigator Award from Subdivision on Chromatography and Separation Chemistry of the Analytical Chemistry Division of the ACS Event Type: Award Event Title: High-throughput, Ultrasensitive Isoelectric Focusing Presider(s): Bidlingmeyer, Brian A Start Time: 10:45 AM ( Slot # 8 ) Date: Wednesday, March 3rd, 2010 Location: 300 Keywords: Capillary Electrophoresis, Fluorescence

Abstract Content

We report a multiplexed capillary electrophoresis system employing an array of 32 capillaries with a micromachined sheath flow cuvette as the detection chamber. The sample streams were simultaneously excited with a 473 nm laser beam, and the fluorescence emission was imaged on a CCD camera with a pair of doublet achromat lens. Reconstructed electropherograms were corrected for migration time drift using a pair of internal standards. The instrument shows sensitivity in the zeptomole range. Isoelectric focusing of fluorescent standards produced peaks with a width of 0.0029 ± 0.0008 pH, and a pair of features associated with myoglobin generated peaks with width of 0.008 ± 0.003 pH; these appear to be among the highest resolution isoelectric focusing separations based on soluble ampholytes. Capillary coating stability limits the reproducibility of the analysis.