Affinity Methods in Biochemical Separations
Wednesday, March 3rd, 2010
2:00 PM Room: 308B

Organizers:
Adam T Woolley, Brigham Young University

Speakers:
2:00 PMIntroductory Remarks -
2:05 PMRecent Advances and Applications of Microaffinity Chromatography, David Hage
2:40 PMStudying Molecular Interactions Using Micro Free Flow Electrophoresis, Michael Bowser
3:15 PMMulti-functional Device Based on Immunoaffinity Capillary Electrophoresis for the Study of Toxicoproteomic Biomarkers, Norberto Guzman
3:50 PMImmunoaffinity Capillary Electrophoresis Using Cleavable Detection Tags, Charles Henry
4:25 PMIntegrated Affinity/Capillary Electrophoresis Microchips for Multiplexed Biomarker Quantitation, Adam Woolley


Overview:
The separation of biomolecules is key in clinical diagnostics, proteomics, and various other –omics approaches. The field of affinity methods, where analysis is enabled by antibodies, aptamers or other molecules with strong binding to a target, is emerging as an important area in biochemical separations. The proposed symposium will feature key advances in affinity methods in biomolecular separations, which are critical in proteomics, drug discovery, ligand design, etc. An excellent lineup of speakers has been assembled. (1) Prof. Michael Bowser from the University of Minnesota will talk about utilizing microfluidic methods in optimizing aptamer binding. (2) Prof. Charles Henry of Colorado State University will present work on novel cleavable fluorescent tags for immunoaffinity capillary electrophoresis in biomedical and environmental applications. (3) Prof. Adam Woolley of Brigham Young University will describe integrated affinity/capillary electrophoresis microchips for point-of-care biomarker detection. (4) Dr. Norberto Guzman of Princeton Biochemicals, who is a pioneer in the field of affinity separations by capillary electrophoresis, will present recent work in applying this method to chronic disease biomarker isolation and characterization. (5) Prof. David Hage of the University of Nebraska will talk about advances and applications of microaffinity chromatography.