Basic Information
Abstract Number: 1200-3    
Author Name: Julie S Biteen Affiliation: University of Michigan
Session Title: Pittsburgh Spectroscopy Award - WEBCASTING
Event Type: Award
Event Title: Live-Cell Single-Molecule and Super-Resolution Imaging in Bacteria
Presider(s): Smith, Joanne H Start Time: 03:20 PM ( Slot # 5 )
Date: Tuesday, March 13th, 2012 Location: 300
Keywords: Biomedical, Fluorescence, Imaging, Microscopy

Abstract Content
Single-molecule fluorescence imaging has improved the resolution of fluorescence microscopy down to the nanometer scale. This super-resolution technique is non-invasive, tolerates simple sample preparation, and takes advantage of high-specificity labeling schemes. We have developed a collection of single-molecule imaging methods that allow us to extend the capabilities of this technique beyond proof-of-concept experiments to answer important problems in microbiology and materials science.

Here, we focus on imaging structure and dynamics in live bacterial cells, with attention to the particular challenges that this system presents: the organisms are small, have short cell cycles, live in particular environments, and their organization is relatively poorly understood. We resolve in two and three dimensions the midplane ring formed by the prokaryotic cell-division protein FtsZ in Caulobacter crescentus using the natural dynamics of the protein (“Dynamics-PALM”). We also apply live-cell single-molecule imaging to two novel problems of great biomedical significance. Investigations of the regulatory pathway controlling pathogenicity in Vibrio cholerae allow insight into the underexplored mechanism of membrane-associated DNA transcription, and studies of the mechanism of carbohydrate catabolism in Bacteroides thetaiotaomicron require oxygen-free methods to investigate this anaerobic gut symbiont.

The methods developed for biological imaging can also be applied to investigations of the of complex nanostructures. Here, we examine the structure and local environment of metal-organic framework crystals by single-particle tracking, and we characterize microfluidic nanochannels with micelle-assisted blink microscopy.