Basic Information
Poster Number: 1110-14    
Author Name: Sureyya Ozcan Affiliation: University of California, Davis
Session Title: Bioanalytical LC-MS I
Event Type: Poster
Event Title: Characterization of Novel Glycans Isolated from Tear and Saliva
Presider(s):   Start Time: ( Slot # 14 )
Date: Tuesday, March 13th, 2012 Location: Red Area on Exposition Floor, Aisles 1300-1500
Keywords: Characterization, Ion Cyclotron Resonance, Liquid Chromatography/Mass Spectroscopy, Tandem Mass Spec

Co-Authors
NameAffiliation
An, Hyun JooChungnam National University
Lebrilla, Carlito BUniversity of California, Davis

Abstract Content
Glycomics is emerging as a simple yet highly sensitive diagnostic tool for disease onset and progression. Human fluids are the most relevant biological source for monitoring actual body status from a glycomics perspective. However, the glycome of human saliva and tear has been little explored in terms of disease marker. In this research, N and O-glycans from tear and saliva samples were profiled and their structures were elucidated using a mass spectrometry-based technique. Then, the glycan profiles were compared in order to determine glycan correlation in saliva and tears. Finally, the N and O-glycan profile found in control tear and saliva was compared to that of patients suffering from ocular rosacea, a form of dry eye disease. These comparisons showed that sulfated acidic O-glycans are major components in diseased patient tears and saliva. On the other hand, highly fucosylated N-glycans were correspondingly abundant in control saliva and tear. Characterization of novel glycans performed by MALDI FTICR and nano LC CHIP QTOF based tandem techniques. The sulfated glycans comprising of mucin core 1 and core 2 type structures were more abundant in the presence of ocular rosacea. Sialylated and unique sulfated-sialylated O-glycans also found in patient tears and saliva whereas they were relatively low abundant compared to sulfated O-glycans. The results indicate that glycomes of saliva and tears show parallel characteristics. The findings demonstrate that patient samples were readily differentiated by a group of N and O-glycans, suggesting their potential as a biomarker for ocular rosacea. Additionally, novel glycans found in tear and saliva are new candidate for disease marker studies.