Basic Information
Abstract Number: 1590-3    
Author Name: Mark C Roman Affiliation: Tampa Bay Analytical Research
Session Title: Addressing Challenges in Dietary Supplement Analysis
Event Type: Workshop
Event Title: Optimization of Chromatographic Selectivity for the Analysis of Dietary Supplements
Presider(s): Rimmer, Catherine A Start Time: 09:15 AM ( Slot # 4 )
Date: Wednesday, March 14th, 2012 Location: 313
Keywords: Chromatography, Food Science, HPLC, Optimization

Abstract Content
The analysis of dietary supplements presents a number of significant challenges. Dietary supplements can be single botanical products, or a complex mixture of botanical extracts, vitamins, and minerals, and can be in tablet, hardshell capsule, softgel capsule, or liquid form. The analytes and potential interferences can cover a broad spectrum of chemical properties and concentration ranges. Good Manufacturing Practices (GMPs) for dietary supplements state that manufacturers must establish product specifications to ensure the identity, strength and composition, along with establishing limits on contaminations/adulterants to help ensure the product’s purity. Chromatographic techniques such as HPLC or GC are commonly used by manufacturers or their contract laboratories to ensure these specifications are met. Obtaining adequate selectivity between the actives/marker compounds of interest, and/or known and unknown interferences can be challenging. A variety of tools, including the use of different column chemistries, mobile phase properties, high efficiency stationary phase particles, and temperature are at the chromatographer’s disposal. Systematic optimization studies can help achieve the required selectivity and resolution to ensure the reliable quantitation of ingredients or marker compounds in dietary supplements.