Nitroxide Spin Labels in NMR and ESR Studies of Protein
Wednesday, March 14th, 2012
2:00 PM Room: 207A

Organizers:
Sunil Saxena, University of Pittsburgh

Speakers:
2:00 PMIntroductory Remarks -
2:05 PMConformational Sampling of HIV-1 Protease by Pulsed EPR Spectroscopy, Gail Fanucci
2:40 PMProtein Fold Determined by Paramagnetic Magic-Angle Spinning Solid-State NMR Spectroscopy, Christopher Jaroniec
3:15 PMProtein Conformational Dynamics from Spin Labeling EPR Spectroscopy, Hassnane Mchaourab
3:50 PMHydration Dynamic Landscape of Proteins and Lipid Membranes by Overhauser Dynamic Nuclear Polarization, Songi Han
4:25 PMThe Dynamics and Orientations of Spin Labeled Side Chains in the Restriction Endonuclease EcoRI and Protein-G, Sunil Saxena


Overview:
The use of site-directed spin-labeling has revolutionized the use of ESR and NMR to provide exciting new methodologies for measuring folding patterns and conformational dynamics in proteins inaccessible by other biophysical methods. The talks will highlight the applications in systems such as membrane proteins, ion-channels, misfolded or fibrillar proteins. The symposium will also present new methodology for the quantification of local dynamics, large distances, as well as novel hyperpolarization methods to enhance the signal in NMR by the use of nitroxide spin labels.