|Abstract Number: ||1320 - 5|
|Author Name: ||Adam B Hall - Boston University School of Medicine|
|Session Title: ||New and Emerging Analytical Technologies in Forensic Science|
|Event Type: ||Symposia|
|Event Title: ||High Throughput Analytical Separations of Drugs of Abuse and Their Metabolites by Differential Mobility Spectrometry - Mass Spectrometry (DMS-MS)|
|Presider Name:||Ruth Smith||Co-Author:||Stephen L Coy, Erkinjon G Nazarov, Paul Vouros|
|Affiliation:||Michigan State University||Affiliation:||Northeastern University, University of Southern Florida|
|Date: ||Tuesday, March 19, 2013|
|Start Time: ||03:50 PM (Slot #5)|
|Forensic drug analysis and toxicology laboratories are inundated with thousands of samples requiring chromatographic separations for the isolation of analytes of interest prior to mass spectrometry. In an effort to conduct rapid ion separations and characterization of a wide array of analytes including drugs of abuse and their metabolites, two, planar, differential ion mobility spectrometry (DMS) – mass spectrometry (MS) platforms have been developed.|
Differential mobility employs asymmetrical electrical fields to accomplish the separation of diverse ion populations within the gas phase. The development and optimization of robust, high throughput, analytical methods for both qualitative and quantitative forensic applications will be demonstrated during this presentation. Highlights of the experimental findings will be presented in three primary sections: (1) Interfacing DMS to MS, (2) The significance of transport gas modifiers, and (3) Rapid separation and quantitation of drugs of abuse and their metabolites.
A key specific aim of this research has been the development of a high throughput analytical platform for the analysis of drugs of abuse and their metabolites with the overall goal of developing rapid, quantitative techniques in an effort to address an urgent need to reduce case backlogs at crime laboratories nationwide. As compared to traditional GC-MS and LC-MS based techniques requiring 10-45 minutes of chromatography, our research findings have demonstrated that DMS-MS methodologies are capable of separating street quality drug mixtures and drugs metabolites from urine samples in under one minute of total analysis time. High throughput analytical methods are significant to the broader scientific community and can be applied to a wide variety of analytical problems, especially for targeted analyses.