ABSTRACT

Basic Information

Abstract Number: 550 - 3
Author Name: Andrew J deMello - ETH Zürich
Session Title: Emerging Platforms for Lab-on-a-Chip Analyses
Event Type: Symposia
Event Title: High-Throughput Microfluidic Experimentation One Drop at a Time

Presider Name:Ryan T Kelly
Affiliation:Pacific Northwest National Laboratory

Date: Monday, March 7, 2016
Start Time: 02:10 PM (Slot #3)
Location: B301

Abstract Content

The relevance of microfluidic technology is modern experimental science is significant and driven by including the ability to process ultra-small volumes of fluid, enhanced analytical performance, reduced instrumental footprints, facile integration of functional components and the capacity to exploit atypical fluid behaviour to control chemical and biological entities in both time and space. Droplet-based microfluidic systems allow the generation and manipulation of discrete pL-volume droplets contained within an immiscible continuous phase. They leverage immiscibility to create discrete volumes that reside and move within a continuous flow. Such segmented-flows allow for the production of monodisperse droplets at rates in excess of tens of KHz and independent control of each droplet in terms of size, position and chemical makeup. Moreover, the use of droplets in complex chemical and biological processing relies on the ability to perform a range of integrated, unit operations such as droplet generation, merging/fusion, sorting, splitting, dilution, storage and sampling. I will provide examples of how droplet-based microfluidic systems can be used to perform a range of chemical and biological experiments (including nanomaterial synthesis and cell-based assays) in a rapid and efficient manner and also introduce the use of stroboscopic epifluorescence imaging in the extensive characterization of enzyme–inhibitor reaction kinetics and high-throughput imaging flow cytometry.