ABSTRACT

Basic Information

Abstract Number: 2370 - 6
Author Name: Shana Kelley - University of Toronto
Session Title: SEAC - Nanoengineered Biosensors
Event Type: Symposia
Event Title: Ultrasensitive Biomolecular Detection Using Nanostructured Microelectrodes

Presider Name:Reginald M Penner
Affiliation:University of California Irvine

Date: Thursday, March 10, 2016
Start Time: 03:35 PM (Slot #6)
Location: B314

Abstract Content

The analysis of panels of molecular biomarkers offers valuable diagnostic and prognostic information for clinical decision making. Robust, practical platforms that detect low levels of biomolecules (< 1000 copies) are urgently needed to advance medical care by diagnosing and predicting the progression of cancer and other disease states. Electrochemical methods providing low cost and direct biomarker read-out have attracted a great deal of attention for this application, but have, to date, failed to provide clinically-relevant sensitivity. We exploit controlled nanostructuring of electrode surfaces to promote surface accessibility and enhance capture rate and efficiency to solve this long-standing problem, and showed that the nanoscale morphologies of electrode surfaces control their sensitivities. In addition, we have worked towards integrating nanomaterials-based electrodes into a chip-based platform to facilitate multiplexed analysis in a robust, practical format. Recently, we have developed assays that are able to detect nucleic acids, proteins and small molecules, with universally high sensitivity levels. This presentation will highlight how the nanostructured electrodes were engineered to exhibit high levels of sensitivity and specificity, and how they have been integrated with devices for sample preparation and data analysis.