ABSTRACT

Basic Information

Abstract Number: 1870 - 3
Author Name: Richard M Crooks - University of Texas at Austin
Session Title: Electrical and Electrochemical Sensing and Detection based on Nucleic Acid Recognition
Event Type: Symposia
Event Title: Detection of Hepatitis B Virus DNA with a Paper Electrochemical Sensor

Presider Name:Rebecca LaiCo-Author:Xiang Li, Karen Scida
Affiliation:University of Nebraska-LincolnAffiliation:The University of Texas at Austin

Date: Wednesday, March 9, 2016
Start Time: 02:10 PM (Slot #3)
Location: B309

Abstract Content

In this presentation we show that a simple paper-based electrochemical sensor, fabricated by paper folding, is able to detect a 30-base nucleotide sequence characteristic of DNA from the hepatitis B virus (HBV) with a detection limit of 85 pM. This device is based on design principles we have reported previously for detecting proteins via a metalloimmunoassay. It has four desirable attributes. First, its design combines simple origami (paper folding) assembly, the open structure of a hollow-channel paper analytical device to accommodate micron-scale particles, and a convenient slip layer for timing incubation steps. Second, two stages of amplification are achieved: silver nanoparticle labels provide a maximum amplification factor of 250,000 and magnetic microbeads, which are mobile solid-phase supports for the capture probes, are concentrated at a detection electrode and provide an additional ~25-fold amplification. Third, there are no enzymes or antibodies used in the assay, thereby increasing its speed, stability, and robustness. Fourth, only a single sample incubation step is required before detection is initiated.