Course Information
Course Title: Ion Mobility Spectrometry with Mass Spectrometry
Categories: 1 - Atmospheric Pressure Ionization
2 - Liquid Chromatography-Mass Spectrometry
3 - Mass Spectrometry
4 - Chiral Separations
5 - Proteomics
6 - Quality/Regulatory/Compliance
7 - Enantiomeric Separations
8 - Environmental Analysis
9 - Gas Chromatography-Mass Spectrometry
10 - Sensors
11 - Homeland Defense
12 - Ion-Mobility Spectrometry
Instructor(s): Herbert Hill, Steve Harden Course Number: 95
Affiliation: WSU
Course Date: 03/10/2009 - Tuesday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $425 ($625 after 2/9/09) Textbook Fee:

Course Description
We are proposing a full day session on ion mobility spectrometry. The sessions will be arranged such that stand alone ion mobility spectrometry (IMS) will be discussed in the first session and ion mobility coupled with mass spectrometry (IMMS) will be discussed in the second session. It is envisioned that each session will stand alone. Thus it could be viewed (or listed) as Ion mobility spectrometry as a ½ day short course followed by another ½ day short course called “ion mobility coupled mass spectrometry”. Ion Mobility Spectrometry (IMS) has come of age in the 21st century as an analytical detection and separation method. Applications of IMS and IMS - Mass Spectrometry (IMS-MS) are expanding rapidly – from sensors for explosive and chemical warfare agent detection to rapid methods for separation of complex biological mixtures before MS. Today, there are a number of commercial IMS instruments available of various designs and objectives. Airports use IMS instruments for detecting explosives and government agencies carry IMS instruments on raids for on-site identification of drugs of abuse. On-site monitoring of pesticides, chemical warfare agents and industrial chemicals are common. IMS is no longer limited to gas phase samples as the introduction of electrospray as an ionization source has enabled IMS to be used for the analysis of aqueous samples. Development of high-resolution ion mobility spectrometry has led to the rapid separation of complex mixtures and isomeric compounds. Extension of IMS as a tool for rapid analysis into areas such as drug discovery and molecular biology is truly exciting. This basic course introduces the analytical applications of ion mobility for separation and measurement of a wide variety of chemicals. The primary objective of the course will be to offer sufficient theoretical and practical information to evaluate IMS for real-world applications. Discussions of instrumental designs, ionization sources, modes of operation, chemical interferences, resolution, sensitivity, detection limits, and dynamic response ranges will provide a knowledge base from which to select an instrument and method that will best accomplish a desired measurement

Target Audience
The target audience are scientists who have purchased or who are considering purchasing an ion mobility spectrometer or an ion mobility mass spectrometer. The course will be conducted at the beginner level assuming no knowledge of ion mobility spectrometry but some knowledge of analytical chemistry, chromatography and mass spectrometry.

Course Outline
Session I:  Ion Mobility Spectrometry
1. Introduction
o Overview of applications
2. Types of Ion mobility spectrometry
o Time-of-flight
 Ambient pressure
 Low pressure
o Field Asymmetric Ion Mobility Spectrometry
o Deferential Mobility Spectrometry
o Aspirating Ion Mobility Spectrometry
o Hyphenated Methods
3. Ionization Sources used in IMS
o Radioactive sources
o Corona discharge 
o Photo ionization 
o Electrospray ionization
4. Figures of Merit
o Sensitivity
o Detection limit
o Dynamic response range
o Selectivity
o Resolving power
5. Parametric affects on Figures of Merit
o Temperature
o Pressure
o Electrical potential
o Geometry
6. Applications
o Explosives detection
o Chemical warfare agent detection
o Drug detection
o Drug clean-up validation
o Environmental analysis
Session II: Ion Mobility coupled with Mass Spectrometry
1. Review of Ion Mobility Spectrometry
a. Time-of-flight Ion Mobility Spectrometry
b. Field Asymmetric Ion Mobility Spectrometry
2. Types of Ion Mobility Mass Spectrometry
a. IMS-Quad MS
b. IMS-Ion Trap MS
i. Electrospray ionization
1. low pressure ion mobility 
2. Ambient pressure ion mobility
3. Applications
a. Proteomics
b. Metabolomics
c. Glycomics
d. Gas Phase Chiral Separations
e. Security
i. Explosives
ii. Chemical warfare agents
iii. Biological warfare agents
f. Environmental analysis

Course Instructor's Biography
Dr. Hill obtained his Ph.D. degree in Chemistry in 1975 from Dalhousie University of Halifax, Nova Scotia, under the direction of W.A. Aue. While at Dalhousie, he was a member of the Trace Analysis Research Centre and was awarded the Izaak Walton Killam Memorial Scholarship for advanced study. Before joining the chemistry faculty at WSU in 1976, he was a postdoctoral fellow in the laboratory of F. W. Karasek at the University of Waterloo where he first demonstrated the applicability of ion mobility spectrometry for the trace detection of organic compounds such as drugs and explosives. During this time he also demonstrated the first use of selective reactant gases for IMS and identified reactant and product ions using ion mobility mass spectrometry. During the 1980s he continued his work with IMS developing the IMS designs which are currently used today and interfacing IMS to various forms of chromatography. During the 1983-84 academic year he received a 10-month fellowship from the Japan Society for the Promotion of Science (JSPS) for research at Kyoto University, Kyoto, Japan. In 1989 received the Keene P. Dimick award in Chromatography for his development of electrospray ion mobility spectrometry and the use of IMS as a chromatographic detector. Throughout the 1990s he has continued to develop IMS using electrospray ionization for the detection of chemical warfare agents, explosives and drugs as well as developing high resolution IMS coupled with mass spectrometry for the separation and detection of biological compounds. In recent years his interest has been in the determination of interferences to the IMS detection of explosives. He has demonstrated the used of IMMS for the separation and detection of blood metabolites and has developed a method to separate chiral compound in the gas phase by ion mobility spectrometry. He has over 200 peer reviewed scientific publications; several patents related to IMS; and two edited books on trace detection and chromatographic detectors. At Washington State University he has served as director of the Office of Grants and Research Development, Associate Dean of the Graduate School, and is currently a full Professor in the department of Chemistry. In 2007 he was selected as the Distinguished Faculty of Science at Washington State University.