Course Information
Course Title: Molecular and Particle Characterization by Dynamic Light Scattering and Zeta Potential
Categories: 1 - Life Sciences
2 - Pharmaceutical Sciences
3 - Data Analysis
4 - Polymers
5 - Environmental Analysis
6 - Food
Instructor(s): Ana Morfesis, Ulf Nobbmann Course Number: 152
Affiliation: Malvern Instruments
Course Date: 03/16/2011 - Wednesday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $225 ($325 after 2/14/11) Textbook Fee:

Course Description
The objectives of this short course are to discuss dynamic light scattering (DLS, PCS, QELS), molecular weight and electrophoretic light scattering (zeta potential) measurements. We will review dynamic light scattering theory, molecular weight determinations, provide useful tips for sample preparation and analysis of measurement results. DLS is a versatile and non-destructive measurement technique that is ideally suited as a metrology of choice for size, stability and identification of protein molecules, aggregates and particles over a wide range of materials in solution. We will also discuss electrophoretic light scattering measurements and the importance of zeta potential in understanding interfacial behaviour and the formulation of multi- component products.

Target Audience
Formulation scientists involved in the characterization of proteins, biomolecules, liposomes, polymers, metal oxides, nanotechnology and other material research applications. Anyone working in a field or application area that requires an understanding of particle size, molecular weight and formulation stability.

Course Outline
2:00 – Introduction & Course Objectives
     2:10 - Dynamic Light Scattering (DLS) & Molecular Weight: Theory and
   Data Analysis
     2:50 - Zeta Potential: Electrophoretic Light Scattering (ELS)
   Measurements and Theory
     3:40 – Break
     4:00 – Dynamic Light Scattering & Molecular Weight: Applications and
   Data Interpretation
     4:40 – Zeta Potential: Particle Interactions and Formulation Stability
     5:30 – Questions
     5:45 – End

Course Instructor's Biography
Ulf Nobbmann, Ph.D. in Physics, Oklahoma State. Dr. Nobbmann is a light scattering specialist for Malvern Instruments. His research has focussed in the areas of light scattering from biological molecules and nano particles. Ana Morfesis, Ph.D. in Physical Chemistry, University of Massachusetts-Amherst. Dr. Morfesis is a light scattering specialist for Malvern Instruments. Her research has focussed in the areas of light scattering and zeta potential of nano and bio materials.