Course Information
Course Title: Techniques for the Handling of Biological Samples I
Categories: 1 - Life Sciences
2 - Atomic Force Microscopy
3 - Biomedical Engineering
4 - Microscopy
5 - Sensors
6 - Laboratory-on-a-chip/Microfluidics
Instructor(s): Jaime Castillo Course Number: 66
Affiliation: Technical University of Denmark
Course Date: 03/10/2012 - Saturday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $235 ($335 after 2/13/12) Textbook Fee: $135 (Same Text as Course 67)

Course Description
Several micro- and nanomanipulation techniques have emerged in recent decades thanks to advances in micro- and nanofabrication. For instance, the atomic force microscope (AFM) uses a nano-sized tip to image, push, pull, cut, and indent biological material in air, liquid, or vacuum. Using micro- and nanofabrication techniques, scientists can make manipulation tools, such as microgrippers and nanotweezers, on the same length scale as the biological samples. This course reviews the different techniques available to manipulate and integrate biological materials in a controlled manner, either by sliding them along a surface (2-D manipulation), or by gripping and moving them to a new position (3-D manipulation). The advantages and drawbacks are mentioned together with examples that reflect the state-of-the-art in manipulation techniques for biological samples.

Target Audience
Anyone working within biomedical engineering, chemical engineering, chemistry and biochemistry, physicians). Scientist doing research in the areas of biomaterials, cell handling, bionanotechnology, drug delivery, and tissue engineering, regenerative medicine. Nanotechnology students.

Course Outline
Effects of scaling down on biological samples
Manipulation using Scanning Probe Microscopy
Manipulation by electric fields
Optical manipulation techniques

Course Instructor's Biography
Jaime Castillo graduated in Chemistry in 2000 at the Industrial University of Santander (Colombia). He joined the biosensor research group at the Biotechnology Department at Lund University (Sweden) in 2001 where he carried out his PhD research entitled “Amperometric biosensors for detection of analytes in cellular models”. In 2006 he worked as a postdoctoral fellow at the Elektroanalytik & Sensorik group at the Analytical Chemistry Department at Bochum University (Germany), where he was working in the fabrication and development of biosensors for the detection of compounds of biomedical relevance in cells using Scanning Electrochemical Microscopy (SECM). In 2007 he joined the Micro and Nanotechnology Department, DTU Nanotech, at the Technical University of Denmark (DTU) as a postdoctoral fellow. In 2009 he was appointed as assistant professor at the same department. At DTU Nanotech he is a member of the Nano Bio Integrated Systems group (NaBIS). Jaime has been supervising a number of master and PhD students. He has been involved in several European research projects, published several articles in various journals and international conference proceedings and contributed to one textbook. His research focuses in the manipulation and integration of biological nano structures into new innovative ways of designing and assembling man-made devices such as nanosensors or drug delivery systems. Winnie Svendsen was born in Copenhagen, Denmark in 1966. She received her B.Sc degree in 1992 and M.Sc. degree in physics in 1993 from the University College Dublin, Ireland; here she received the EOLAS applied research award for excellent research. Her Ph.D. was from Copenhagen University and RISŲ and was finalized in 1996. In 1996 she accepted a post. doc position at the Max Planck Institute for Plasma physics. In 1998 Winnie received a talent stipend form SNF (now FNU) and the prestigious Curie stipend from Copenhagen University to establish a research group to design a hyperpolarized gas set-up for use in medical lung diagnostic. In 1999 she was appointed associate professor at Copenhagen University. She was the co-founder of a company XeHe Hypol (APS). Since 2000 Winnie has been employed as associated professor at DTU. In 2006 she established her own research group Nano Bio Integrated Systems (NaBIS). Winnie has a thorough knowledge on biosensing using nanotechnology and fabrication.