Short Course Listings
Short Course

Course Information
Course Title: Introduction to LCMS for Chromatographers and Beginners
Categories: 1 - Liquid Chromatography-Mass Spectrometry
Instructor(s): Robert Classon Course Number: 118
Affiliation: Shimadzu
Course Date: 03/21/2013 - Thursday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $480 ($680 after 2/18/13) Textbook Fee:

Course Description
This is an introductory course in LCMS for people who have not done LCMS previously. It covers the practical aspects of LCMS presented at a level that novices can understand. The focus is on understanding the 'what, where, how and why' of LCMS including real world examples at each step. It takes a new user through all the aspects of understanding the 'buzzwords', figuring out what is important, how to deal with the chemistry issues relating to mass spec, and how to interpret the results. From a practical aspect, it illustrates how to avoid the pitfalls and problems most common for new users of LCMS such as how to prepare a sample for LCMS analysis, how to develop a LCMS friendly method, and how to get good results on the first try. No prior knowledge about mass spectrometry or LCMS is required.

Target Audience
Anyone using HPLC now, or doing chemical research, pharmaceutical analysis, food chemistry, organic synthesis, environmental analysis, QC, structural analysis, etc. will benefit from this course. The course shows how to deal with complex samples, improve sensitivity or selectivity, speed up analyses, and identify contaminants and unknowns.

Course Outline
• Basic types of LC/MS
• Why LCMS – Advantages of LC/MS over Other HPLC Detectors
• What Data and Information Can be Cbtained by LC/MS
• Empirical Formula Determination
• Scan Types and Uses
• Making Ions
• Chemistry Issues with LCMS
• How do Interfaces/Sources for LCMS Work
• Separating Ions – Mass Analyzers for LC/MS
• Detecting Ions
• Chemistry and Chromatography for LC-MS
• Sample Cleanup Techniques
• Practical LCMS Method Development
• Converting HPLC Methods to LCMS Methods
• Plumbing issues
• Qualitative versus Quantitative aspects of LC/MS
• Installation Requirements for LC/MS
• Understanding Product Specifications
• Preventive Maintenance

Course Instructor's Biography
Robert Classon - 40+ years practical usage and engineering experience with HPLC, 15+ years with LCMS,conducted numerous seminars and workshops on practical aspects of LCMS, member Editorial Board for Journal of Instrumentation Science & Technology Ross Willoughby - Former VP R&D for Extrel, 35+ years practical usage and R&D of LC/MS, developer of particle beam mass spec, inventor with >40 patents for LC/MS, Coauthor of “A Global View of LC/MS: How to solve your most challenging analytical problems”, Research interests are currently in AP-optics and field-free sources for LC/MS, 10+ years experience in applications development