Short Course Listings
Short Course

Course Information
Course Title: Advances in MS and LC/MS Sample Introduction, Sample Placement and Liquid Handling
Categories: 1 - Liquid Chromatography-Mass Spectrometry
2 - Mass Spectrometry
3 - Sample Preparation
4 - Forensics
5 - Homeland Defense
6 - Laboratory-on-a-chip/Microfluidics
Instructor(s): Drew Sauter, Alexander Scheeline Course Number: 58
Affiliation: nanoLiter LLC
Course Date: 03/04/2014 - Tuesday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $270 ($375 after 2/10/14) Textbook Fee:

Course Description
This course is for those interested in mass spectrometry sample introduction and sample preparation, as induction based fluidics (IBF) does both and more. It is for experienced mass spectroscopist and novices their managers and QA officials, as we demonstrate applications and capabilities using videos. After this course, the attendees will never view liquids, electric fields and MS sample introduction and preparation in the same way.

Target Audience
The target audience is for mass spectroscopist of all levels and those doing sample preparation and sample introduction and their mangers and QA officials. Attendees will not only learn about our latest sample improvements published in Analytical Chemistry, JASMS, Polymer and RCMS, they will learn how electic fields can be used to fly liquids for many purposes including 3D printing.

Course Outline

Overview of IBF and Applications.

Videos of IBF
ESI w/100% MS sample introduction efficiency.
LC/MALDI with msec. depositions.
3D-Print, PVA.
IBF and Mixing.
nanoLiter dispensing, a demonstration, all participate.
The chemistry and physics of ESI, IBF contrasted.
Trajectories of drops. Thermodynamics.
Directing, measuring drops to targets.
Introduction summary

Peer Review Paper Application Review. IBF: ESI, MALDI, SIMS, LDI other techniques.
Why nanoliters?
Excellent crystals for MALDI.
100% efficient ESI, IBF yields standard ESI mass spectra.
Parallel dispensing.
Viscous liquids, whole blood, serum, glues.
Quick summary and peer review papers.
Dispensing: Video of making one channel and 384 channel depositions.
MALDI (peptides,proteins, polymers,cells)
SIMS (explosives, drugs)
Direct ESI analysis of mixtues: urine;blood;coffee; cells liquids;more.
Other Apps: TLC,IR Spec, Chemical warfare, HLS, etc.
Review of mass spectra with study, Organics, Inorganics, Organometallics.

nanoLiter Cool Wave MS, dispensers, LC's, syringes, pipettes, embodiments.
Cool video demo (Flying nLs into levitated uLs!)
Energy regime
Femtogram and attogram sensitivity.
Practical sample intro/handling issues.

New developments.
3D Printing appications for MS, TLC,MALDI.
Millisecond sample analysis
N=10 or 20 multiplexing.
Single molecule?
Future, a new very low cost dispenser.

Course Instructor's Biography

Drew Sauter invented and patented induction based fluidics(IBF). It is the fastest, most efficient (100%) technique for launching samples into LC/ESI/MS systems and for LC/MALDI. IBF can also be used for parallel dispensing and more including LO 3D printing. Drew founded nanoLiter LLC and has collaborated with over fifty groups worldwide on IBF. Sciex offered to license the technology and devices have been sold to US Army, Genentech, Biogen Idec, Amgen, NIH, NIST, U’s of CA,WI, ILL and 50 other corporations and other organizations. America’s greatest mass spectroscopist has called our ability to “shoot” 100% of cellular liquids using IBF into ANY ESI, “exciting,” and IBF as “great.”

Prior to this work, Drew developed MS methods and technology in support of defense, energy and environmental projects at MRI. He developed the core GC/MS methods used environmentally across the USA since the 70's. He was on the team that built the first national database of pollutants from 20,000 data files of USA rivers publishing on signal processing, pattern recognition and database building. Later he purchased Dr. Robert Finnigan's first TSQ and did the first LC/MS/MS HTS in the 80's, hands on. He directed MS/MS R&D with Hunt and Shabanowitz and directed seminal funding to Dr. S. Houk, the inventor of ICP/MS. He acquired funding for Extrel and Dr. Willoughby to develop new LC/MS technology that was purchased by Waters, that being their entree' into MS.

As a consultant, he was hired by USDOJ and played a key role in winning a nine-figure litigation against 20 of the 40 largest firms in the USA using applied multivariate statistics and pattern recognition. For WMI, he developed an economic model for a $20M lab which paid for itself in under three years as he predicted. In the 90's he won a $M contract at Los Alamos, for building a data management systems for 34 instrumental chemistry techniques that also addressed data integrity.

Then, after reading ca. 600 papers on ESI over a two year period, he invented and patented IBF, a technique that improves MALDI and allows for 100% of liquids to be "shot" into any ESI or LC/ESI. This technology he predicts will replace the spray part of ESI. It is IBF that is at the core of this course.

Drew founded nanoLiter LLC and and sells devices and licenses technology. He has worked with firms on four continents, and been hired as a consultant by most MS firms for business issues of MS to LC/MALDI. He has also been hired as an expert in MS by seven different branches of the US government, and he has given numerous invited presentations and written twelve patents, hundreds of reports, peer review and other papers, our most recent ten being addressed in this course.He holds an MS degree from Marquette and a BS degree from Duquesne University both in chemistry.

Alexander Scheeline is Professor of Chemistry Emeritus at the University of Illinois at Urbana Champaign, Vice President for Engineering of Anchor Science LLC, and co-founder of SpectroClick Inc. In the first of these roles, he and his students have used induction based fluidics to study chemical kinetics in ultrasonically-levitated drops. In the second role, he is helping develop and market products in thermal management. Finally, in the third, he is developing spectrometers that exploit the strengths and overcome the limitations of consumer CMOS cameras for performing spectrophotometry and spectrofluorimetry.