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Short Course

Course Information
Course Title: Introduction to Metabolomics
Categories: 1 - Atmospheric Pressure Ionization
2 - Life Sciences
3 - Liquid Chromatography-Mass Spectrometry
4 - Mass Spectrometry
5 - Pharmaceutical Sciences
6 - Sample Preparation
Instructor(s): Dajana Vuckovic Course Number: 25
Affiliation: Concordia University
Course Date: 03/02/2014 - Sunday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $500 ($710 after 2/10/14) Textbook Fee:

Course Description
This course will provide an introduction to various global and targeted metabolomics approaches using liquid chromatography – mass spectrometry. The course will cover practical and fundamental aspects of sample preparation, LC-MS analysis, experimental design, quality control, data processing, statistical interpretation including mulitivariate statistical analysis and metabolite identification strategies in the context of global metabolomics. Examples from various fields including biomedical, clinical, life sciences will be highlighted throughout the course. Strategies to enhance metabolite coverage and data quality for a given application will be discussed in detail. Practical examples and critical discussion will be incorporated throughout the course to deepen the participants’ understanding of the field of metabolomics and understand current advantages and limitations of various metabolomic approaches.

Target Audience
This introductory course is intended for professionals in analytical chemistry and/or life sciences to learn the basics of LC-MS metabolomics research for various applications including biomedical, clinical, biological, toxicological, food and pharmaceutical. The participants are expected to have basic knowledge of separation theory and mass spectrometry.

Course Outline
1. Introduction to metabolomics and types of applications, definitions, overview of analytical methodology
2. Sample preparation methods in global metabolomics for cells, biological fluids, tissues and plants
3. Separation methods in global metabolomics: reversed-phase, HILIC, CE
4. Mass spectrometry methods in global metabolomics: high-resolution and mass accuracy
5. Experimental design considerations and quality control procedures
6. Brief overview of common data processing and interpretation strategies including metabolite databases and multivariate statistical analysis such as principal component analysis
7. Introduction to lipidomics
8. Targeted metabolomics: class-specific approaches
9. Example case studies: critical discussion of selected metabolomics studies to highlight good and poor design strategies and advantages/limitations of various metabolomics approaches
10. Future perspective

Course Instructor's Biography
Dr. Dajana Vuckovic is an assistant professor in the Department of Chemistry and Biochemistry at Concordia University, Montreal, Canada. She holds Honours B.Sc. in Chemistry from the University of Toronto and Ph.D. in Analytical Chemistry from the University of Waterloo. Her doctoral research with Prof. Janusz Pawliszyn focused on the development of an innovative in vivo solid-phase microextraction methodology for global metabolomics using liquid chromatography - high resolution mass spectrometry, while her postdoctoral research at the University of Toronto with Prof. Andrew Emili focused on new chemical proteomics workflow for the determination of protein targets of drugs and natural products. She is the recipient of several awards including NSERC Postdoctoral Fellowship and 2010 Douglas E. Ryan Graduate Student Award by Canadian Society for Chemistry. To date she has authored 27 publications, 8 book chapters and 45 conference contributions. She currently serves as the Editor of Sample Preparation, an Editorial Board member of Bioanalysis and Associate Editor of Journal of Integrated Omics. She is a member of Canadian and American Chemical Societies, American Society for Mass Spectrometry, Metabolomics Society and Canadian National Proteomics network. At Concordia, she is currently establishing a state-of-the-art research program in analytical and clinical metabolomics with particular focus on the development of new strategies to improve metabolome coverage of unstable and low abundance metabolites and the development of improved diagnostic methods.