Short Course Listings
Short Course

Course Information
Course Title: Separations: Fundamentals of Advanced Gel Permeation and Size Exclusion Chromatography Detection
Categories: 1 - Analytical Metrology
2 - Life Sciences
3 - Liquid Chromatography
4 - Pharmaceutical Sciences
5 - Polymers
6 - Quality/Regulatory/Compliance
Instructor(s): Ulf Nobbmann Course Number: 133
Affiliation: Malvern
Course Date: 03/12/2015 - Thursday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $270 ($375 after 2/20/15) Textbook Fee:

Course Description
This 1/2 day course covers principles of advanced detection in GPC & SEC: Conventional chromatography relies solely on a concentration detector (RI, UV) to find Mw, Mn, PDI, %aggregation, by linking the elution to similar known standards. Universal Calibration avoids the need for similarity, exploiting the universal scaling of intrinsic viscosity. Light scattering provides the ‘absolute’ molecular weight directly. The different calibration techniques (conventional, universal, light scattering) will be covered with examples. The details of intrinsic viscosity and light scattering detection will include basics (principle of operation, math, caveats) and applications in real laboratory research & QAQC scenarios.

Target Audience
Those with some general practical GPC or SEC experience who want to maximize the value obtained from their existing advanced detectors and those who are considering the potential benefit of adding such to their chromatography characterization portfolio: - Scientists, R&D personnel - Laboratory Technicians - Quality assurance and quality control staff

Course Outline
1:00pm ‐ 1:15pm Introduction & Course Objectives: What is advanced GPC/SEC?
1:15pm ‐ 1:45pm Fundamentals of Conventional Calibration Chromatography
1:45pm ‐ 2:15pm Fundamentals of Universal Calibration Chromatography & Intrinsic Viscosity
2:15pm ‐ 2:30pm Coffee/Tea Break
2:30pm ‐ 3:15pm Fundamentals of Light Scattering: RALS, LALS, MALS
3:15pm ‐ 4:15pm Case Studies of Applications in Polymers, Biopolymers & Proteins
4:15pm ‐ 5:00pm Practical Take Home Messages, Question and Answer Session

Course Instructor's Biography
Ulf Nobbmann, Ph.D. in Physics, Oklahoma State. Dr. Nobbmann is Nanometrics Product Manager – Americas for Malvern Instruments. He has extensive experience in light scattering as a tool for chemical, biochemical and biophysical laboratories.