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Short Course

Course Information
Course Title: Comprehensive Two-Dimensional Liquid Chromatography
Categories: 1 - Life Sciences
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Data Analysis
5 - Food
Instructor(s): Dwight Stoll, Peter Carr Course Number: 108
Affiliation: Gustavus Adolphus College
Course Date: 03/07/2015 - Saturday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $500 ($710 after 2/20/15) Textbook Fee:

Course Description
In this course we will provide a survey of the most important theoretical and practical issues to consider when developing two-dimensional liquid chromatography methods. We will begin with a discussion of the potential advantages of 2D methods over 1DLC separations, both for targeted and comprehensive 2D analyses. We will provide an overview of current understanding of factors that affect 2D separation performance, including the impacts of undersampling and orthogonality of separation mechanisms. We will summarize best practices for implementation of 2D separations in application areas including food and beverage analysis, pharmaceutical separations, and metabolomics. Finally, we will discuss current approaches to quantitation and visualization of 2D data, and review the characteristics of commercially available instrumentation for 2D separations.

Target Audience
The target audience is people who are either considering implementing two-dimensional separations in their laboratories, or have already purchased and instrument are seeking to quickly learn the fundamentals of the technique and best practices for method development to make the most of their effort with the approach.

Course Outline
1. Theoretical advantages of 2DLC over 1DLC
2. Theoretical considerations for implementation of 2DLC
A. Impact of first dimension undersampling on 2D separation performance
B. Separation orthogonality and separation mechanisms
C. Optimization of performance
3. Practical factors for implementation of 2DLC
A. Compatibility of first and second dimension separations
B. Optimization of instrument configuration for low level detection
C. Influence of pumping system design and operation on 2D separation speed and performance
4. Data analysis in 2DLC
A. Approaches to quantitation and visualization of 2D chromatograms
B. Overview of advanced chemometric treatment of 2D data
5. Survey of commercially available instruments and components for 2DLC
6. Q & A

Course Instructor's Biography
The instructors will be: Dwight Stoll, Ph.D. - Associate Professor of Chemistry, Gustavus Adolphus College Peter Carr, Ph.D. - Professor of Chemistry, University of Minnesota Together the instructors have published over 30 papers in the area of two-dimensional liquid chromatography in the last decade, including several review articles and book chapters. During the same time they have given over 50 conference presentations and organized several symposia and tutorials on the subject at meetings including Pittcon and the HPLC20XX series. Most recently, Dr. Stoll taught a one-day short courses at the HPLC2013 conference in Amsterdam (June, 2013), which was very well attended with 60 participants, and at Pittcon 2014. We have seen a dramatic increase in interest in 2DLC from non-expert users in the past two years, and anticipate that there will be continued and growing interest in this topic in the short course format.