Short Course Listings
Short Course

Course Information
Course Title: Two-Dimensional Liquid Chromatography for Pharmaceutical Analysis
Categories: 1 - Life Sciences
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Chemometrics
5 - Pharmaceutical Sciences
6 - Proteomics
Instructor(s): Dwight Stoll, Kelly Zhang Course Number: 139
Affiliation: Gustavus Adolphus College
Course Date: 03/06/2016 - Sunday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $300 ($400 after 2/12/16) Textbook Fee:

Course Description
In this course we will provide a survey of important theoretical and practical issues to consider when developing two-dimensional liquid chromatography methods for pharmaceutical analysis. We will begin with a discussion of the potential advantages of 2D methods over 1DLC separations, and show examples of 2D methods for solving real analytical problems in pharmaceutical analysis. We will discuss heartcutting, comprehensive, and hybrid modes of 2D separation, and both small and large molecule analyses. We will provide an overview of current understanding of factors that affect 2D separation performance, including the impacts of undersampling, orthogonality of separation mechanisms, and detection sensitivity. Finally, we will discuss current approaches to quantitation and visualization of 2D data, and review the characteristics of commercially available instrumentation for 2D separations.

Target Audience
The target audience is people who are either considering implementing two-dimensional separations for pharmaceutical analysis, or have already purchased and instrument are seeking to quickly learn the fundamentals of the technique and best practices for method development to make the most of their effort with the approach.

Course Outline
1. Theoretical advantages of 2DLC over 1DLC
A. Overview of heartcutting, comprehensive, and hybrid 2D separations
B. Review of advantages of 2D for small and large molecule separations
2. Theoretical considerations for implementation of 2DLC
A. Impact of first dimension undersampling on 2D separation performance
B. Separation orthogonality and separation mechanisms
C. Impact of method development decisions on detection sensitivity
D. Optimization of performance
3. Practical factors for implementation of 2DLC
A. Compatibility of first and second dimension separations
B. Optimization of instrument configuration for low level detection
C. Influence of pumping system design and operation on 2D separation speed and performance
4. Data analysis in 2DLC
A. Approaches to quantitation and visualization of 2D chromatograms
B. Overview of advanced chemometric treatment of 2D data
5. Survey of commercially available instruments and components for 2DLC
6. Q & A

Course Instructor's Biography
The instructors will be: Dwight Stoll, Ph.D. - Associate Professor of Chemistry, Gustavus Adolphus College Kelly Zhang, Ph.D. - Genentech Together the instructors have published over 25 papers in the area of two-dimensional liquid chromatography in the last decade, including several review articles and book chapters. Dr. Zhang is a recognized leader in the adoption of 2D-LC in the pharmaceutical industry. During the same time they have given over 40 conference presentations and organized several symposia and tutorials on the subject at meetings including Pittcon and the HPLC20XX series. Most recently, Dr. Stoll taught one-day short courses at the HPLC2013 conference in Amsterdam, at Pittcon 2014, at Pittcon 2015, and a tutorial at the HPLC2015 meeting in Geneva. We have seen a dramatic increase in interest in 2D-LC in the pharmaceutical industry in the past few years, and anticipate that there will be continued and growing interest in this topic in the short course format.