Short Course Listings
Short Course

Course Information
Course Title: Highly Successful Strategies for LC/MS Quantitation: Current Applications and Emerging Technologies
Categories: 1 - Atmospheric Pressure Ionization
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Mass Spectrometry
5 - Clinical Analysis
6 - Pharmaceutical Sciences
Instructor(s): Rick King Course Number: 46
Affiliation: Pharmacadence Analytical Services, LLC
Course Date: 03/08/2016 - Tuesday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $550 ($750 after 2/12/16) Textbook Fee:

Course Description
This course will provide a comprehensive survey of the current LC/MS-based approaches for quantitation. Instrumentation, strategies, and methods will be presented with specific focus on: sample preparation, chromatography, ionization, and mass spectrometry. The balance between high performance and high throughput will be critically examined using real-world examples. Straightforward tips for dealing with carry over and matrix effects, two common problems associated with LC/MS approaches for quantitation, will be described. Emerging trends for high performance quantitation that involve the use ultra high performance liquid chromatography, nanospray, and accurate mass analysis techniques will be discussed.

Target Audience
The purpose is to assist scientists with knowledge and practical skills leading to the generation of reliable information in a research or academic setting. The course is ideal for a beginner who is relatively new to the field or an expert/manager who is in need of an updated perspective on current strategies and emerging analytical technologies.

Course Outline
Lecture 1. Introduction to Quantitation
- Overview
- Analytical Figures of Merit
- Industry Perspectives

Lecture 2. LC/MS Overview
- Chromatography Considerations – Sample Preparation and Analytical
- Method Development
- Ionization
- Mass Analysis

Lecture 3. Instrumentation
- Sample Handling
- Chromatography
- Mass Spectrometry
- Integration

Lecture 4. Chemistry, Chemistry, Chemistry – A Reversed Engineered Solution
- Sample Preparation
- Analytical Chromatography
- Ionization

Lecture 5. Case Studies and Examples
- Case Study I (perhaps historical – hassan fouda?)
- Case Study II (perhaps middle of the road & updated – merck/king)
- Case Study III (current example which we admire – qual/quan from Kevin bateman)

Lecture 6. Method Development & Troubleshooting
- Carry Over
- Matrix Effects – Sea Monsters!
- Industry Perspectives

Lecture 7. Emerging Trends
- Ultra High Performance Liquid Chromatography
- Nanospray
- Accurate Mass Analysis

Course Instructor's Biography
Dr. Richard C. King has over 16 years of industrial experience in the pharmaceutical industry. He was active in the field of mass spectrometry and he led preclinical drug metabolism studies at Merck Research Laboratories from 1994-2008. An expert in bioanalysis and the integration of new technologies into an industrialized workflow, Dr. King has participated in the growth of quantitative mass spectrometry via his work on ion formation mechanisms, ion chemistry, separation sciences, automation, and data processing. He obtained his Ph.D. in analytical chemistry from Drexel University under the direction of Professor Kevin Owens. Dr. King founded PharmaCadence, a preclinical contract research organization, in 2008. Dr. Mike S. Lee has extensive experience with pharmaceutical analysis and drug development. He has pioneered the application of LC/MS in different phases of drug development for research in biomolecules, natural products, drug metabolites, impurities, and degradants. Dr. Lee was with Bristol-Myers Squibb from 1987-1998. As a director of analytical research & development, Dr. Lee was responsible for departments in several research facilities providing MS, NMR, IR, HPLC, CE, and physical chemistry support. He obtained his Ph.D. in analytical chemistry from the University of Florida under the direction of Professor Richard A. Yost. Dr. Lee has been president of Milestone Development Services since 1998 and actively participates in the growth of new technologies and their integration into drug development.