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Short Course

Course Information
Course Title: An Introduction to Mass Spectrometry Including Biomolecule Applications
Categories: 1 - Atmospheric Pressure Ionization
2 - Liquid Chromatography-Mass Spectrometry
3 - Mass Spectrometry
4 - Proteomics
5 - Gas Chromatography-Mass Spectrometry
6 - Teaching Analytical Chemistry
Instructor(s): Bryan Ham Course Number: 95
Affiliation: U.S. Customs and Border Protection
Course Date: 03/08/2016 - Tuesday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $550 ($750 after 2/12/16) Textbook Fee: $115 and $155

Course Description
The objective of the course is to introduce the fundamentals of mass spectrometry including ionization sources, mass analyzers, reaction rate theory, and spectral interpretation. The course also includes examples of the application of mass spectrometry to biomolecule analysis including lipids, carbohydrates, nucleic acids, and proteomics. The aim of the course is to provide a basic understanding of the fundamentals of mass spectrometry and its application to biomolecule analysis.

Target Audience
Chemists, lab technicians, analysts and undergraduate and graduate students studying the sciences. The course will appeal to anyone who is interested in having a survey of an introduction to mass spectrometry all the way through to the application to biomolecules.

Course Outline
The proposed course is a survey of the fundamentals of mass spectrometry including ionization techniques such as electron ionization, electrospray, and MALDI, mass analyzers such as quadrupole, TOF, FTICR, and Orbitrap, reaction rate and collision theory, and product ion spectral interpretation thus providing an overall introduction to mass spectrometry and its use in biomolecule analysis. The course is designed to teach the fundamentals of mass spectrometry along with the basic tools needed for spectral interpretation using examples from biomolecule analysis including lipids, carbohydrates, nucleic acids, and proteomics. The course will draw from the author’s book “Even Electron Mass Spectrometry with Biomolecule Applications.”

Introduction and Fundamentals
Mass Spec Definition
The Mass Spectrum
Peak Resolution
Mass Accuracy
Ionization in Mass Spectrometry
Odd Electron Ionization
Even Electron Ionization
Mass Analyzers in Mass Spectrometry
Product Ion Spectra
Product Ion Spectral Interpretation
Biomolecule Spectral Interpretation – Small Molecules
Fatty Acids
Wax Esters
Sterols
Acylglycerols
ESI-Mass Spectrometry of Phosphorylated Lipids
Biomolecule Spectral Interpretation - Biological Macromolecules
Carbohydrates
Nucleic Acids
Proteins

Course Instructor's Biography
Dr. Bryan Ham has worked in analytical chemistry laboratories for over 20 years including petrochemical, environmental, foodstuff, and life sciences research. He has published 15 research papers in peer reviewed journals and two books: Even Electron Mass Spectrometry with Biomolecule Applications, John Wiley & Sons, Inc., 2008, and Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques, John Wiley & Sons, Inc., 2012. He has a third book under contract to be published shortly entitled Analytical Chemistry: A Chemist and Laboratory Technician’s Toolkit, John Wiley & Sons, Inc., 2015. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection New York Laboratory. He is currently a member of the American Society of Mass Spectrometry (ASMS) and the American Chemical Society (ACS). His research interests include the application of mass spectrometry for biomolecular analysis in the areas of proteomics, lipidomics, and metabolomics, and foodstuff chemistry.