Short Course Listings
Short Course

Course Information
Course Title: Essentials of Modern HPLC/UHPLC 2: Operation, Troubleshooting, and Method Development
Categories: 1 - Life Sciences
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Pharmaceutical Sciences
5 - Quality/Regulatory/Compliance
Instructor(s): Michael Dong Course Number: 4
Affiliation: MWD Consulting
Course Date: 03/06/2016 - Sunday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $550 ($750 after 2/12/16) Textbook Fee: $70

Course Description
This intermediate course will introduce you to best practices of high-performance liquid chromatography (HPLC) and ultra-high-pressure liquid chromatography (UHPLC) as well as tricks-of-the-trade and best practice for successful HPLC operation. The attendees will learn step-by-step guide to operating HPLC modules, maintenance procedures, troubleshooting strategies, traditional and accelerated method development processes as well as recent advances of this analytical technique including a section on UHPLC. This is the second part of a two-course series for modern HPLC introduction. “Essentials of Modern HPLC/UHPLC 1” introduces HPLC fundamental concepts, columns, instrumentations and applications, is a prerequisite.

Target Audience
Analysts, scientists, researchers and managers who want to get an intermediate course on modern HPLC practices, instrument operation, maintenance, troubleshooting, method development and UHPLC. A good understanding of general chemistry is recommended. Some prior hands-on HPLC experience will be helpful but not mandatory.

Course Outline
5. HPLC operation, maintenance and troubleshooting
• Mobile phase preparation
• Best practice in HPLC operation
• Guidelines for increasing HPLC precision and for trace analysis
• Common HPLC maintenance procedures
• Problem diagnosis and troubleshooting guide with case studies
• Diagnosing and solving problems (pressure, baseline, peak, data performance)

6. HPLC method development and validation
• Tradition strategy for HPLC method development
• 3-pronged template approach for efficient method development
• Method prequalification and validation
• Modern trends – software tools and automated systems
• Case studies on developing ICH impurity testing of pharmaceuticals including some discussions on phase-appropriate method development concepts

7. Ultra-high pressure LC 1 – concepts, perspectives, and benefits
• History and current status of UHPLC
• Practical concepts and instrumental considerations
• Benefits: very fast separation with good resolution, very high resolution of
complex samples, and facilitating rapid method development
• Case studies (NCE, OTC, complex molecules and natural products)

8. Ultra-high pressure LC 2 – practice, potential issues and QC implementation
• Potential issues and operating nuances (viscous heating, system dispersion, compatibility to existing methods, injection precision and carryover, detector sensitivity and dwell volumes, and method transfer issues)
• Case studies illustrating issues and myths, and how to mitigate potential pitfalls
• How to get started and to transition from HPLC to UHPLC

Course Instructor's Biography
Dr. Michael W. Dong is a principal in MWD Consulting focusing on training and consulting services in HPLC/UHPLC, pharm analysis and drug quality. He was formerly Senior Scientist at Genentech, Research Director at Synomics Pharma, Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems / Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from City University of New York, and a certificate in Biotechnology at U. California. Santa Cruz. He has over 100 publications and a best-seller book in HPLC. He is an editorial advisory board member of LCGC magazine and American Pharmaceutical Review.