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Short Course

Course Information
Course Title: Introduction to Quantitative Real-Time PCR
Categories: 1 - Life Sciences
2 - Clinical Analysis
3 - Polymerase Chain Reaction
Instructor(s): Mikael Kubista Course Number: 120
Affiliation: TATAA biocenter
Course Date: 03/07/2016 - Monday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $550 ($750 after 2/12/16) Textbook Fee:

Course Description
Molecular diagnostics and molecular research are experiencing a technology shift. A new platform based on ultra sensitive detection of nucleic acids - called quantitative real-time PCR (qPCR) - has become available that makes it possible to determine the amounts of specific nucleic acids and even proteins in biological samples with unsurpassed accuracy and sensitivity. This has opened new possibilities to detect pathogens in biological and environmental samples and to study and diagnose complex diseases. This 1-day course will give participants an overview of real-time PCR technology and its possibilities, as well as insight in the future development of the technology platform and forthcoming applications.

Target Audience
This course is intended for researchers and laboratory personnel working with or planning to work with real-time PCR, researchers with interest in gene and protein expression, clinicians and pathologists with interest in molecular diagnostics, virologists, bacteriologists, and scientists with interest in food diagnostics and environmental science.

Course Outline
• Brief historic survey of PCR
• Basics of real-time PCR, applications and possibilities of the technology
• Present and forthcoming instrumentation platforms
• Probing technologies
• Multiplex qPCR
• Design and performance of qPCR experiments.
• Data pre-treatment and basic analysis
• Normalization strategies
• Quantification strategies
• Extraction of DNA and RNA
• Reverse transcription
• Experimental design and quality assessment
• Mutation and methylation analysis using HRM
• Immuno qPCR for protein detection
• qPCR expression profiling
• Digital PCR
• Quality assessment and quality control
• The MIQE guideline

Course Instructor's Biography
Dr Kubista is founder and CEO of the service provider and course organizer TATAA Biocenter, of the data analyses company MultiD Analyses, and of the diagnostic company Life Genomics. He was one of the pioneers contributing to the development of quantitative real-time PCR (qPCR) and introduced qPCR for single cell expression profiling and mutlimodal profiling. He led the development of reagents for high throughput expression profiling and quality control at TATAA Biocenter and he developed qPCR tomography for intracellular expression profiling. Kubista coauthored the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. TATAA was member of the SPIDIA effort that generated data for the forthcoming CEN technical specifications and ISO guidelines and Kubista is member of the technical committees drafting the guidelines. Kubista is scientific advisor to Roche and ThermoFisher Scientific.