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Short Course

Course Information
Course Title: Separations: Fundamentals of Advanced Gel Permeation & Size Exclusion Chromatography Detection
Categories: 1 - Analytical Metrology
2 - Life Sciences
3 - Liquid Chromatography
4 - Pharmaceutical Sciences
5 - Polymers
6 - Quality/Regulatory/Compliance
Instructor(s): Ulf Nobbmann Course Number: 113
Affiliation: Malvern
Course Date: 03/09/2016 - Wednesday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $300 ($400 after 2/12/16) Textbook Fee:

Course Description
This day course covers principles of advanced detection in GPC & SEC: Conventional chromatography relies solely on a concentration detector (RI, UV) to find Mw, Mn, PDI, %aggregation, by linking the elution to similar known standards. Universal Calibration avoids the need for similarity, exploiting the universal scaling of intrinsic viscosity. Light scattering provides the ‘absolute’ molecular weight directly. The different calibration techniques (conventional, universal, light scattering) will be covered with examples. The details of intrinsic viscosity and light scattering detection will include basics (principle of operation, math, caveats) and applications in real laboratory research & QAQC scenarios.

Target Audience
Those with some general practical GPC or SEC experience who want to maximize the value obtained from their existing advanced detectors and those who are considering the potential benefit of adding such to their chromatography characterization portfolio: - Scientists, R&D personnel - Laboratory Technicians - Quality assurance and quality control staff

Course Outline
(15min) Introduction & Course Objectives: What is advanced GPC/SEC?
(30min) Fundamentals of Conventional Calibration Chromatography
(30min) Fundamentals of Universal Calibration Chromatography & Intrinsic Viscosity
(15min) Coffee/Tea Break
(45min) Fundamentals of Light Scattering: RALS, LALS, MALS
(60min) Case Studies of Applications in Polymers, Biopolymers & Proteins
(45min) Practical Take Home Messages, Question and Answer Session

Course Instructor's Biography
Ulf Nobbmann, PhD in Physics, Oklahoma State. Dr. Nobbmann is GPC/SEC & Nanometrics Product Manager – Americas for Malvern Instruments. He has extensive experience in light scattering as a tool for chemical, biochemical and biophysical laboratories.