Short Course Listings
 
Short Course

Course Information
Course Title: Immunoanalytical Methods for Environmental, Food and Clinical Analysis
Categories: 1 - Environmental Analysis
2 - Food
3 - Sensors
4 - Water-Wastewater
5 - Laboratory-on-a-chip/Microfluidics
6 - Life Sciences
Instructor(s): Rudolf Schneider Course Number: 151
Affiliation: BAM
Course Date: 03/07/2017 - Tuesday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $325 ($425 after 2/18/17) Textbook Fee:

Course Description
The Short Course offers an introduction into antibody-based analytical techniques and is suitable also for beginners. It comprises Lecture Units teaching the concepts, equipment, reagents and explains the protocols to be used in the practical part. Simple “Hands On” units are offered employing portable instrumentation. Topics span from antigen production, generation of antibodies, assay development and optimization, data evaluation to the many different existing formats. Examples are provided from the areas of environmental analysis (pharmaceuticals, hormones and anthropogenic markers in water and wastewater), food analysis (caffeine in beverages, mycotoxins) and clinical diagnostics (infarction biomarkers).

Target Audience
Scientists, especially analysts as well as (under)graduate students from the areas (bio)chemistry, environmental engineering, food science, forensics. Open for lab technicians. The course appeals to anyone who wants or needs to explore the advantages that immunochemical methods present for the environmental/Food/clinical lab – and to learn about pitfalls.

Course Outline
LECTURES:
1) Immunoanalytical Methods – an overview
2) Generation of polyclonal and monoclonal antibodies
3) Obtaining antibodies for analytes of low molecular weight (haptens)
o Hapten design and synthesis
o Conjugates for immunoanalytical methods
4) Immunoassays
o Colorimetric Enzyme Immunoassay (ELISA)
o Data structure, sensitivity and selectivity, matrix effects
o Non-colorimetric ELISA (chemiluminescent and other substrates)
o Employment of magnetic beads
o Homogeneous formats (e.g. Fluorescence Polarization
Immunoassay, FPIA)
5) Microfluidic formats, lab-on-chip/disc/valve, immunosensors
o Electrochemical immunoassays
o Lateral-flow Immunoassays (LFIA)
6) Microarrays
o Flat arrays
o Bead-based arrays
7) Immunoaffinity columns

Hands-On:
1) Working with immunoaffinity columns
2) Cocaine detection on banknotes by ELISA (magnetic beads, tubes)
3) Quantification of caffeine in beverages by FPIA
4) Detection of Bisphenol A (BPA) with a Dipstick Assay (LFIA)
5) Biochips (examples, including arrays for heart infarction markers)

(Hands-on is generously supported by instrumentation loan of Abraxis Inc., Warminster, PA, USA)

Course Instructor's Biography
Rudolf J. Schneider has more than 25 years of experience in development and application of antibody-based analytical methods such as immunoassays (ELISA) and other immunochemical formats with over 70 research papers. - He received his diploma in Chemistry and PhD in Analytical Chemistry (1993) from Technical University of Munich, Germany, then became a researcher and Lecturer at the University of Bonn where he received his habilitation (2004) in Environmental Chemistry/Agro-Ecology (2004). Post-doctoral stays in Portugal, the USA and Italy. In 2005 he joined the Analytical Chemistry Department of BAM Federal Institute for Materials Research and Testing in Berlin, first as a group leader and later as Head of Division “Environmental Analysis”. Since 2010 he is also Lecturer for “Biochemical Environmental Analysis” at Technical University of Berlin. He has mentored more than 15 PhD students and is teaching Immunoanalytics to students at all levels. Co-Instructor: Peter Carl, chemistry studies at Humboldt University of Berlin, PhD student at BAM with the topic “Immunomicroarrays for environmental applications”.