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Short Course

Course Information
Course Title: Fast GC Analysis: How to Achieve Fast Separation to Increase Sample Throughput and Productivity
Categories: 1 - Gas Chromatography
2 - Gas Chromatography-Mass Spectrometry
Instructor(s): Yelena Sapozhnikova Course Number: 129
Affiliation: USDA
Course Date: 03/09/2017 - Thursday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $325 ($425 after 2/18/17) Textbook Fee:

Course Description
This course will provide theoretical fundamentals of low pressure (LP) vacuum outlet gas chromatography (GC) as well as practical hands-on experience on LPGC assembly and set up that can be implemented in any laboratory for fast separation of semi-volatile organic compounds. While fast LC ˗ UPLC has been around since 2004, fast alternatives for GC separation are available, but hugely under-utilized. In the instructor’s laboratory, LPGC is routinely used for separation of ≈300 compounds in 10 min, including late eluting pyrethroid pesticides and brominated flame retardants. The main advantage of LPGC is speed, which provides 4 times faster separation compared to conventional GC, thus leading to high sample throughput and productivity. Just imagine getting results 4 times faster! Additional LPGC benefits include greater sensitivity and sample loadability (lower LOQs), lower elution temperature, leading to less degradation for thermally liable compounds, greater ruggedness and reduced peak tailing. Thus far, LPGC has been successfully used for analysis of pesticides and metabolites, PAHs and other hydrocarbons, PCBs, PBDEs and other flame retardants, phytosterols, explosives, organotins, plant oils, and fragrances. In short, LPGC can increase productivity, reduce time needed for GC separation, improve instrument ruggedness and reduce time for maintenance.

Target Audience
This course is for anyone using GC-MS or GC-MS/MS in their laboratory. Any laboratory personnel, scientists interested in speeding up GC separation for semi-volatile organic compounds (pesticides, environmental contaminants, nutraceutical, forensic, petroleum applications) will benefit from this course. Note: course level - any

Course Outline
Theory of fast low pressure (vacuum outlet) chromatography Use of analyte protectants to reduce matrix effects Comparing conventional GC with LPGC: pros and cons Real world examples of LPGC applications in different areas of analysis Overview of practical considerations Hands-on practice to LPGC column assembly and installation

Course Instructor's Biography
Dr. Yelena Sapozhnikova is a Research Chemist with the United States Department of Agriculture (USDA) in Wyndmoor, PA. Her research focuses on developing new and improving existing analytical methods for fast, efficient, high-throughput analysis of pesticides and organic environmental contaminants using LC- and GC-MS/MS techniques. Dr. Sapozhnikova has over 20 years of GC experience, including 5 years using fast LPGC.