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Short Course

Course Information
Course Title: Basic HPLC - Fundamentals, Applications and Troubleshooting
Categories: 1 - Food
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Pharmaceutical Sciences
5 - Quality/Regulatory/Compliance
6 - Sample Preparation
Instructor(s): Fredric Rabel Course Number: 55
Affiliation: ChromHELP LLC
Course Date: 03/06/2017 - Monday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $575 ($775 after 2/18/17) Textbook Fee:

Course Description
This course covers the basics of high performance liquid chromatography (HPLC). All of the components of the HPLC system from the pumps to the detectors will be discussed. Troubleshooting of potential or often seen problems will be shown, whether they are the fault of the LC system, the column, or the user. The modes of chromatography, related columns and mobile phases used for various applications will be discussed with emphasis on the reversed phase mode. Basic mobile phase and sample preparation, and column care (including protector columns) will be taught since these are all critical in giving optimum method reproducibility and column life. Attendees are invited to bring their problem HPLC methods or chromatograms for discussion.

Target Audience
This course is of interest to anyone who will be doing hands-on HPLC who have not used it before or are relatively new to it. The course is designed to give an understanding of the basic principles of theory and operation to allow a HPLC method to be set up and used successfully. NOTE - this is a classroom setting so NO hands-on is possible.

Course Outline
Morning
Chromatography theory and terminology
HPLC system components
Modes of chromatography
Optimizing column configurations for speed
Bonding chemistry and the newest LC packings for fast HPLC
Afternoon
Using the reversed phase mode
Using the hydrophilic interaction mode (HILIC)
Care and maintenance of LC columns
Troubleshooting separation problems
Internet and other help sources

Course Instructor's Biography
Fred Rabel is the President of ChromHELP, LLC, consulting and teaching all of the liquid chromatography techniques (TLC, HPLC, Column Chromatography, and Sample Preparation). He previously worked for J.T.Baker Chemical Co., Whatman Inc., and EMD Chemicals. He is on the editorial boards of Journal of Liquid Chromatography and Related Techniques, and Journal of Chromatographic Science. He has been teaching HPLC courses for over 35 years. He has authored many chromatography articles and chapters.