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Short Course

Course Information
Course Title: Basic HPLC Method Development
Categories: 1 - Food
2 - Liquid Chromatography
3 - Liquid Chromatography-Mass Spectrometry
4 - Pharmaceutical Sciences
5 - Quality/Regulatory/Compliance
6 - Sample Preparation
Instructor(s): Fredric Rabel Course Number: 56
Affiliation: ChromHELP LLC
Course Date: 03/07/2017 - Tuesday Course Length: 1/2 Day Course
Start Time: 01:00 PM End Time: 05:00 PM
Fee: $325 ($425 after 2/18/17) Textbook Fee:

Course Description
This course will cover the basics of method development for an analytical separation. The process begins with outlining the needs of the method being developed so more or less attention is paid to the most important details. The details of the needs are then merged with the mode to be used, and any related history that is known or can be found in the literature. This gets the method development off to a faster, more confident start. The method is completed by optimizing factors of column selectivity, column size and flow rates to give a rapid, reproducible final method. This course will cover the basics of method development for an analytical separation. The process begins with outlining the needs of the method being developed so more or less attention is paid to the most important details. The details of the needs are then merged with the mode to be used, and any related history that is known or can be found in the literature. This gets the method development off to a faster, more confident start. The method is completed by optimizing factors of column selectivity, column size and flow rates to give a rapid, reproducible final method.

Target Audience
This course was designed for those who have been using HPLC but now need to begin to develop new methods or optimize on older methods currently in use. Today through the use of newer columns and LC systems considerable analytical time (and money) savings can be accomplished. Also important is making the method user friendly by testing within the laboratory.

Course Outline
HPLC analytical method development - what it is
Analysis of the method needed - simple or complex
In-house or on-line help to shorten the process
Choosing initial conditions to begin
Optimizing the method for maximum throughput
Validation of the method
Changing existing methods
Discussion of optimization software

Course Instructor's Biography
Fred Rabel is the President of ChromHELP, LLC, consulting and teaching all of the liquid chromatography techniques (TLC, HPLC, Column Chromatography, and Sample Preparation). He previously worked for J.T.Baker Chemical Co., Whatman Inc., and EMD Chemicals. He is on the editorial boards of Journal of Liquid Chromatography and Related Techniques, and Journal of Chromatographic Science. He has been teaching HPLC courses for over 35 years. He has authored many chromatography articles and chapters.