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Short Course

Course Information
Course Title: Introduction to the Chromatography of Proteins, Peptides, and Related Molecules
Categories: 1 - Liquid Chromatography
2 - Liquid Chromatography-Mass Spectrometry
3 - Pharmaceutical Sciences
4 - Life Sciences
Instructor(s): Thomas Wheat Course Number: 114
Affiliation: Waters
Course Date: 03/04/2017 - Saturday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $575 ($775 after 2/18/17) Textbook Fee:

Course Description
This full day program is an introduction to the separation techniques used to characterize biological macromolecules, with an emphasis on practical applications. The most useful modes for protein separations will be emphasized, and substantial description of peptide mapping will be presented. Topics include theory and principles of the separations. Rationale for choosing a technique will be systematically considered. Strategies for methods development will be presented. Analytical techniques that are often used in the same laboratories, including amino acid, glycan, and oligonucleotide analysis, will be introduced. The role of mass spectrometry in the analytical biochemistry laboratory will be described.

Target Audience
This is a beginner level course. It presumes some training and experience in analytical chemistry. Prior exposure to other separation techniques is useful. It is intended for scientists starting to analyze biological macromolecules and biopharmaceuticals. It surveys the range of available separation techniques to provide a basis for selecting one.

Course Outline
I. Introduction to Separation of Biological Macromolecules

II. Separation of Intact Proteins
A. Size Exclusion Chromatography
B. Ion Exchange Chromatography
C. Reversed-phase Chromatography
D. Hydrophobic Interaction Chromatograhy

III. Peptide Mapping
A. Rationale and purpose
B. Developing a reversed-phase peptide mao
C. Considerations for LC/MS peptide mapping

IV. Other bioseparation techniques
A. Amino Acid Analysis
B. Analysis of protein oligosaccharides
C. Analysis of synthetic oligonucleotides

Course Instructor's Biography
Thomas E. Wheat is a Principal Scientist with Waters, a supplier of instrumentation and chemistry for UPLC, HPLC and Mass Spectrometry. He earned a Ph.D. in Cell Biology from the University of Illinois. He has used and studied protein and peptide chromatography for over 35 years