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Short Course

Course Information
Course Title: Analytical Organic Mass Spectrometry
Categories: 1 - Liquid Chromatography-Mass Spectrometry
2 - Mass Spectrometry
3 - Gas Chromatography-Mass Spectrometry
Instructor(s): William Budde Course Number: 71
Affiliation: Retired USEPA
Course Date: 03/07/2017 - Tuesday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $575 ($775 after 2/18/17) Textbook Fee:

Course Description
This is not an introductory course. Attendees who lack adequate background and experience will likely be lost after the first hour. This course is intermediate between an introductory course and a specialized course on one specific topic. The course focuses on those practical techniques and strategies that are needed to conduct organic chemical analyses with gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC)/MS). The topics include a brief description of six standard ionization techniques, a brief non-mathematical description of four types of mass spectrometers, four key mass spectrometer capabilities, important mass spectrometry limitations, and some aspects of quantitative analyses. Most topics are discussed in some detail and at an intermediate level with active participation by attendees encouraged. Throughout the course the general interpretation of GC/MS and LC/MS chromatograms and mass spectra is emphasized, with special attention on what to look for in spectra and chromatograms and the analytical information that can be obtained from them. The objective of the course is to provide a reasonably broad, yet in-depth review and/or instruction in organic mass spectrometry with emphasis on the analytical aspects and the capabilities of GC/MS and LC/MS. A detailed discussion of the theory, construction, or principles of operation of mass spectrometers, GC, and LC is beyond the scope of this course. It is assumed that participants have a general knowledge of contemporary GC and/or LC, and some practical experience with GC/MS and/or LC/MS.

Target Audience
This course should be of interest to scientists, chemists, analytical chemists, instrument operators, technicians, laboratory managers, and others who require knowledge of analytical organic mass spectrometry. Participants should have taken an introductory course in mass spectrometry, or have the equivalent in practical experience in the laboratory.

Course Outline
A. Brief review – Basics of Mass Spectrometry
B. Ionization techniques for GC/MS and LC/MS
1. Electron ionization (EI) for GC/MS
a. Energetics and the consequences of ionization
b. Identifying molecular ions and unknowns
2. Chemical ionization (CI) for GC/MS
3. Electron capture ionization (ECI) for GC/MS
4. Atmospheric pressure chemical ionization (APCI) for LC/MS
5. Electrospray ionization (ESI) for LC/MS
6. Atmospheric Pressure Photoionization for LC/MS
C. The four basic types of mass spectrometers
D. Key mass spectrometer capabilities
1. Instrument resolving power (R) and resolution (Δm)
2. Highly accurate mass/charge (m/z) measurements
3. Data acquisition strategies
4. Tandem mass spectrometry
E. Quantitative Analysis
F. A few limitations of mass spectrometry
G. Final questions, discussion, and course evaluation

Course Instructor's Biography
William L. Budde, Ph. D. is a retired analytical organic chemist and mass spectrometrist who was employed by the U. S. Environmental Protection Agency at its research and development laboratories in Cincinnati, Ohio from 1971 - 2006. He conducted extensive research to develop and standardize analytical methods for the identification and measurement of organic substances using gas chromatography (GC)/mass spectrometry (MS) and liquid chromatography (LC)/MS. He received a Ph.D. in physical-organic chemistry from the University of Cincinnati in 1963. He is a member of the American Chemical Society and the American Society for Mass Spectrometry. He has lectured widely and published over 70 scientific journal articles and book chapters and two books. He has taught Analytical Organic Mass Spectrometry and related courses at Pittcon and other conferences since 1995