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Short Course

Course Information
Course Title: Light Scattering Techniques for Protein, Polymer, and Nanoparticle Characterization
Categories: 1 - Analytical Metrology
2 - Life Sciences
3 - Liquid Chromatography
4 - Nanoscience
5 - Pharmaceutical Sciences
6 - Polymers
Instructor(s): Sigrid Kuebler Course Number: 50
Affiliation: Wyatt Technology
Course Date: 03/06/2017 - Monday Course Length: 1/2 Day Course
Start Time: 08:30 AM End Time: 12:30 PM
Fee: $325 ($425 after 2/18/17) Textbook Fee:

Course Description
Light scattering techniques are commonly used to characterize proteins, polymers and nanoparticles in terms of their absolute molecular weight, size, conformation and molecular interactions. This seminar will provide an overview of the fundamentals and applications of Multi-Angle Light Scattering (MALS) and Dynamic Light Scattering (DLS). Both techniques can either be applied in stand-alone (batch) mode as well as coupled to liquid chromatography, e.g. size-exclusion chromatography (SEC) or field flow fractionation (FFF). When used with a separation system, light scattering detection can additionally be used for the assessment of size heterogeneity (aggregation and fragmentation), molecular weight distribution, compositional heterogeneity (protein conjugates and copolymers), conformational analysis, and branching.

Target Audience
This is an introductory course, and no previous light scatting experience is required. The course is suited for scientists in the pharmaceutical, chemical, biotechnology and medical device industry, as well as researchers working in academic or government labs who wish to learn how light scattering can help them characterize their proteins and (bio)polymers.

Course Outline
Topic 1: Fundamentals of Light Scattering
- What is Light Scattering?
- Molecular Properties Determined by Light Scattering
- How Molecular Weight, Size (RMS Radius) and Second Virial Coefficients are Measured by MALS
- How the Size (Hydrodynamic Radius) is Measured by DLS

Topic 2: Principal Applications of Light Scattering
- Goals when Characterizing Proteins, (Bio)polymers and Particles
- When to use Batch vs. Online (Chromatography) Methods with Examples
- MALS, DLS or both?

Topic 3: Advanced Applications
- Analyzing the composition of Protein Conjugates and Copolymers
- Determining Molecular Conformation and Branching

Course Instructor's Biography
Dr. Sigrid Kuebler is the Director of Customer Service and a Senior Application Scientist at Wyatt Technology, and has been directing and teaching Wyatt’s Light Scattering University programs for many years. Prior to joining Wyatt, she worked at Symyx Technologies (now Unchained Labs), where she developed various high-throughput screening techniques for materials characterization. She studied Chemistry at the University of Mainz and the University of Toronto. Her doctoral work was performed at the Max-Planck-Institute for Polymer Research.