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Short Course

Course Information
Course Title: An Introduction to Mass Spectrometry including Biomolecule Applications
Categories: 1 - Atmospheric Pressure Ionization
2 - Liquid Chromatography-Mass Spectrometry
3 - Mass Spectrometry
4 - Proteomics
5 - Sample Preparation
6 - Gas Chromatography-Mass Spectrometry
Instructor(s): Bryan Ham Course Number: 22
Affiliation: Department of Homeland Security
Course Date: 03/06/2017 - Monday Course Length: 1 Day Course
Start Time: 08:30 AM End Time: 05:00 PM
Fee: $575 ($775 after 2/18/17) Textbook Fee: $115

Course Description
The objective of the course is to introduce the fundamentals of mass spectrometry including ionization sources, mass analyzers, reaction rate theory, and spectral interpretation. The course also includes examples of the application of mass spectrometry to biomolecule analysis including lipids, carbohydrates, nucleic acids, and proteomics. The aim of the course is to provide a basic understanding of the fundamentals of mass spectrometry and its application to biomolecule analysis.

Target Audience
Chemists, lab technicians, analysts and undergraduate and graduate students studying the sciences. The course will appeal to anyone who is interested in having a survey of an introduction to mass spectrometry all the way through to the application to biomolecules.

Course Outline
The proposed course is a survey of the fundamentals of mass spectrometry including ionization techniques such as electron ionization, electrospray, and MALDI, mass analyzers such as quadrupole, TOF, FTICR, and Orbitrap, reaction rate and collision theory, and product ion spectral interpretation thus providing an overall introduction to mass spectrometry and its use in biomolecule analysis. The course is designed to teach the fundamentals of mass spectrometry along with the basic tools needed for spectral interpretation using examples from biomolecule analysis including lipids, carbohydrates, nucleic acids, and proteomics. The course will draw from the author’s book “Even Electron Mass Spectrometry with Biomolecule Applications.”

Introduction and Fundamentals
Mass Spec Definition
The Mass Spectrum
Peak Resolution
Mass Accuracy
Ionization in Mass Spectrometry
Odd Electron Ionization (GC-MS)
Even Electron Ionization (ESI, MALDI, etc.)
Mass Analyzers in Mass Spectrometry
Quadrupoles, Ion Traps, TOF, FTICR, Orbitrap
Product Ion Spectra
Spectral Interpretation
Small Biomolecule Applications
Fatty Acids
Wax Esters
Sterols
Acylglycerols
Phosphorylated Lipids
Macro Biomolecule Applications
Carbohydrates
Nucleic Acids
Proteins (Top Down and Bottom Up Proteomics)

Course Instructor's Biography
Dr. Bryan Ham has worked in analytical chemistry laboratories for over 20 years including petrochemical, environmental, foodstuff, and life sciences research, and has a doctorate in analytical chemistry. He has published 16 research papers in peer reviewed journals, encyclopedia chapters, and three books: Even Electron Mass Spectrometry with Biomolecule Applications, John Wiley & Sons, Inc., 2008; Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques, John Wiley & Sons, Inc., 2012; Analytical Chemistry: A Chemist and Laboratory Technician’s Toolkit, John Wiley & Sons, Inc., 2015. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection New York Laboratory. He is a member of the American Society of Mass Spectrometry (ASMS) and the American Chemical Society (ACS). His research interests include the application of mass spectrometry for biomolecule analysis in the areas of proteomics, lipidomics, metabolomics, and foodstuff chemistry.