Basic Information
Abstract Number: 1770-4    
Author Name: Nancy L Allbritton Affiliation: University of North Carolina, Chapel Hill
Session Title: Young Investigator Award from Subdivision on Chromatography and Separation Chemistry of the Analytical Chemistry Division of the ACS
Event Type: Award
Event Title: Microtechnologies to Analyze Single Cells
Presider(s): Bidlingmeyer, Brian A Start Time: 10:10 AM ( Slot # 7 )
Date: Wednesday, March 3rd, 2010 Location: 300
Keywords: Bioanalytical, Biomedical, Capillary Electrophoresis, Enzyme Assays

Abstract Content
Microelectrophoretic methods can make direct measurements of the activity of normal and oncogenic kinases in tumor cells. Issues of heterogeneity within cell populations, both in terms of cell type and biochemical behavior, often mandate single-cell studies for accurate determination of kinase activity in tumor cells. Chemical cytometry, the application of high-sensitivity chemical separations to characterize the contents of single or small numbers of cells, is emerging as an important approach for such studies. Electrophoretic separations within a capillary tube or a microfluidic channel combined with laser-induced fluorescence detection are the most sensitive among these methods. For single-cell kinase kinase assays, a fluorescently-labeled kinase substrate (peptide, protein, or lipid) functions as a reporter of the activity of the targeted kinase. The lab is designing and optimizing new reporters to enhance the efficiency and specificity of kinase measurements on single cells. The throughput of the analysis is being enhanced by the development of automated, serial analysis of cells utilizing a capillary as well as fabrication of polymer, microchips for cell lysis and reporter separation.