Basic Information
Abstract Number: 670-3    
Author Name: Deanna R Jones Affiliation: Centers for Disease Control and Prevention
Session Title: Bioanalytical/Pharmaceutical
Event Type: Poster
Event Title: Improved Biomonitoring Method for Multi-elemental Analysis (Cd, Hg, Pb, Se, and Mn) of Whole Human Blood by Inductively Couple Plasma Dynamic Reaction Cell Mass Spectrometry (ICP-DRC-MS)
Presider(s):   Start Time: ( Slot # 3 )
Date: Monday, March 14th, 2011 Location: Red Area on Exposition Floor - Hall B, Aisle 400
Keywords: Biological Samples, Clinical/Toxicology, Elemental Analysis, ICP-MS

Caldwell, Kathleen LCenters for Disease Control and Prevention
Jarrett, Jeff MCenters for Disease Control and Prevention
Jones, Robert LCenters for Disease Control and Prevention

Abstract Content
Biomonitoring population studies need fast, rugged, and sensitive analytical methods to handle the high throughput of samples in complex matrices (e.g. blood, urine) while measuring low concentrations. Improvements to a biomonitoring method were made including expanding the number of analytes, improving intra-sample precision, and reducing the concentration of materials used in diluent and rinse solutions.

The addition of Se and Mn to the method requires the used of the dynamic reaction cell to reduce the background signals for these elements via ion-molecule reactions. Methane gas (CH4) is used to attenuate 40Ar2 for 80Se and oxygen gas (O2) is used to attenuate the background at m/z 55 for Mn. The addition of elements using the dynamic reaction cell does increase the overall analysis time, however with the use of the SC4-FAST autosampler (ESI Scientific, Omaha, NE) the time for sample-to-sample measurement was kept under 5 minutes.

Ruggedness of the method was evaluated by varying instrumental parameters (e.g. cell gas flow rate, RF Power) to simulate potential day-to-day variations. Data for several figures of merit including accuracy, precision and detection limit will be presented. Reduction of concentration of components of the rinse and diluents solutions decreases the cost of the analysis and reduces the input of harsh chemicals into the ICP-MS.