Basic Information
Abstract Number: 220-6    
Author Name: Akimitsu Kugimiya Affiliation: Hiroshima City University
Session Title: New Developments in Analytical Instrumentation and Software
Event Type: Sunday Poster
Event Title: Spectrophotometric Analysis of Glutamine with Enzymatic Reactions
Presider(s):   Start Time: ( Slot # 6 )
Date: Sunday, March 11th, 2012 Location: Valencia Room, W415
Keywords: Amino Acids, Bioanalytical, Biosensors, Enzyme Assays

Funamoto, DaikiHiroshima City University

Abstract Content
Biosensors are powerful tools in clinical, environmental, and food analysis applications because of the rapid and easy detection procedure. The determination of amino acids is greatly important in clinical settings and food technologies such as the measurement of diagnosis of diseases in clinics and the measurement of freshness of foods in food technologies. The conventional methods of detection for amino acids are pre-column labeling for High-Performance Liquid Chromatography (HPLC) of amino acids with fluorescence derivatives, or post-column labeling for HPLC with ninhydrine, but large amounts of organic solvents and an experimental step for labeling amino acids are necessary.

In this study, for the rapid and easy measurement of amino acids, spectrophotometric analysis method of amino acids was developed with enzymatic reactions. Aminoacyl-tRNA synthetases (ARSs) were used as the molecular recognition element for specific recognition of amino acids. The biosensing system for glutamine was constructed with glutaminyl-tRNA synthetase (GlnRS) and other enzymes, and the response to amino acids was evaluated. By using this sensing system, up to 50 uM of glutamine could be measured and elective response to glutamine was shown.

This work was partly supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN) and the Hiroshima City University Grant for Special Academic Research (General Studies).