Basic Information
Abstract Number: 160-7    
Author Name: Jason A Anspach Affiliation: Phenomenex Inc.
Session Title: High-Throughput Chemical Analysis
Event Type: Oral
Event Title: HPLC Columns Made with Core-Shell Particles: An Elegant Solution for Fast HPLC
Presider(s): Lynch, Garry J Start Time: 03:15 PM ( Slot # 8 )
Date: Sunday, March 11th, 2012 Location: 310B
Keywords: High Throughput Chemical Analysis, HPLC, HPLC Columns, Liquid Chromatography

Farkas, TivadarPhenomenex Inc.
Friedlander, GarethPhenomenex Inc.
Layne, Jeff JPhenomenex Inc.
Loo, Lawrence YPhenomenex Inc.

Abstract Content
In the fast paced world of today’s laboratories there is a lot of emphasis to get very high quality data in as short of a period of time as possible. One of the key tools in the modern analytical laboratory is liquid chromatography. While the ability to separate mixtures into individual components is a powerful tool, it is also considered the slow step in chemical analysis. There are several recent advancements in columns and instrumentation to help maintain separation quality while speeding up the analysis. One of the most recent of these developments is the re-emergence of Core-Shell separation media. These particles contain a non-porous core surrounded by a thin porous shell. When fabricated correctly, these particles provide significantly higher plate counts than fully porous materials of similar size, increasing the number of plates per unit column length. With higher plate counts per unit column length, shorter columns can be used to speed up separations without losing plates. Because there is only minor changes in particle size by switching to the core-shell media from a fully porous media of the same size, there is no additional backpressure required to achieve the efficiency benefit. It is therefore possible to switch to a core-shell based particle similar to whatever fully porous particle that the analysis has been performed on, and perform the analysis on the same instrument. Core-Shell medias, especially in the sub 2 m size, are less prone to frictional heating, which has been shown to be detrimental to long columns packed with fully porous sub 2 m materials when run at high speeds. In this presentation we will demonstrate why core-shell particles offer advantages in efficiency, speed, and resistance to frictional heating over the fully porous counterparts in a variety of high speed HPLC applications.